Alberta NINT/14 May 2008

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lab work (SD): Isolated plasmid DNA from culture tubes using QIAprep miniprep standard protocol.  
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lab work (SD):  
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              Isolated plasmid DNA from culture tubes using QIAprep miniprep standard protocol.  
               Digested DNA with EcoRI + PstI.   
               Digested DNA with EcoRI + PstI.   
               Incubated at 37 C for 2 hrs.  Run on 2% agarose gel.   
               Incubated at 37 C for 2 hrs.  Run on 2% agarose gel.   
               Expected fragment lengths: R0010 200 bp, R0011 55 bp, E0433 533 bp, pSB1A2 ~2 kbp.
               Expected fragment lengths: R0010 200 bp, R0011 55 bp, E0433 533 bp, pSB1A2 ~2 kbp.

Revision as of 02:30, 6 June 2008

lab work (SD): 

              Isolated plasmid DNA from culture tubes using QIAprep miniprep standard protocol. 
              Digested DNA with EcoRI + PstI.  
              Incubated at 37 C for 2 hrs.  Run on 2% agarose gel.  
              Expected fragment lengths: R0010 200 bp, R0011 55 bp, E0433 533 bp, pSB1A2 ~2 kbp.
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