Alberta NINT/16 May 2008

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(New page: lab work (JD): PCR of T/A Sequence, Digested T/A Sequence with Pst, Xba, Ran gel electrophoresis.)
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lab work (JD): PCR of T/A Sequence, Digested T/A Sequence with Pst, Xba, Ran gel electrophoresis.
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[[Team:Alberta_NINT/Notebook | < Back to notebook]]
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lab work (SD, JD):  
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              PCR of T/A I oligonucleotide using Term + and Term - primers
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              DNA digest of T/A I with XbaI + PstI to isolate the terminator and establish sticky ends for
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              insertion into plasmid.
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              Ran on 2% agarose gel.
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[[Image:UVP00296annotated.jpg]]

Latest revision as of 21:40, 11 October 2008

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lab work (SD, JD):

             PCR of T/A I oligonucleotide using Term + and Term - primers 
             DNA digest of T/A I with XbaI + PstI to isolate the terminator and establish sticky ends for
             insertion into plasmid.
             Ran on 2% agarose gel.

UVP00296annotated.jpg