Alberta NINT/18 June 2008

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(Difference between revisions)

Revision as of 20:26, 19 June 2008

lab work (SD):

            Results: K102000 + I732018.1 - 134 colonies
                     K102000 + I732018.2 - 125 colonies
                     K102000 (negative control) - 10 colonies
            Based on sequencing results, 6 colonies from K102000 + I732018.2 plate were inoculated into LB + 
            amp50 culture tubes and incubated at 37 C, shaking, overnight.
            10 ul of K102009 overnight tube from 05/06/08 was inoculated into LB + amp50 culture tube and
            incubated at 37 C, shaking, overnight to create fresh cultures for making a glycerol stock.

            For Wayne: Extracted K102011/H+X from gel using QIA quick gel extraction protocol and a min-elute 
            spin column.  DNA was eluted in 15ul Buffer EB.

lab work (JD):

              Miniprep of K102005 from O/N
              Digested K102005 with BamH1 and Nco1
              Ran on 2% agarose gel and cut out desired band
              Gel purified K102005/B+N
              Ligated our freshly cut K102005/B+N to both our old TA0 that was heated at 50C for 10 minutes and our newly cut TA0 heated at 50C for 10 minutes
              Transformed XL1-B cells with these new K102007s
              Plated K102007 cells
              Counted number of colonies on K102003 plates from yesterday: 5 on K102003(1) and 2 on K102003(2) and 2 on negative plate
              Created 7 O/N of these K102003 colonies

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+[[Team:Alberta_NINT/Notebook | < Back to notebook]]
 lab work (SD):
               Results: K102000 + I732018.1 - 134 colonies