"
Alberta NINT/23 July 2008
From 2008.igem.org
< Previous entry | Next entry >
lab work (SD):
Results from 22/07/08 transformation:
LB+amp50 LB+kan35 LB+amp50+kan35
WM >100 >100 1
SD >100 >100 16
Proceeded with B-Gal Activity Assay when cell cultures grown in the 96-well plate from 21/07/08
showed sufficient cell growth after two days at 37 C. Prepared media
(LB+antibiotics+IPTG+glucose/arabinose) and inoculated 1 ml of media with 20 ul of cell cultures
from the 21/07/08 96-well plate. Incubated at 37 C overnight.
Isolated DNA from overnights of K102011, K102034, K102035, and K102036, using 3 ml to pellet cells
using QIAprep spin miniprep standard protocol (to obtain more DNA for these constructs).
