Alberta NINT/30 May 2008

From 2008.igem.org

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lab work (SD): DNA from LB + amp50 culture tubes (from 29/05/08) was isolated using QIA prep standard miniprep protocol.  This DNA was set up for sequencing. K102002.4 becomes K102002 and K102004.4 becomes K102004.
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lab work (SD):  
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<br><br>
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lab work (JD): Counted colonies on ligation plates: negative plate:0, Positive plate:2 and because only two colonies grew we plated a second positive plate from our O/Ns and left it to grow
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          DNA from LB + amp50 culture tubes (from 29/05/08) was isolated using QIA prep standard miniprep protocol.   
 +
          This DNA was set up for sequencing. K102002.4 becomes K102002 and K102004.4 becomes K102004.
 +
 
 +
 
 +
lab work (JD):  
 +
 
 +
          Counted colonies on ligation plates: negative plate:0, Positive plate:2  
 +
          because only two colonies grew we plated a second positive plate from our O/Ns and left it to grow

Revision as of 21:46, 6 June 2008

lab work (SD):

         DNA from LB + amp50 culture tubes (from 29/05/08) was isolated using QIA prep standard miniprep protocol.  
         This DNA was set up for sequencing. K102002.4 becomes K102002 and K102004.4 becomes K102004.


lab work (JD):

          Counted colonies on ligation plates: negative plate:0, Positive plate:2 
          because only two colonies grew we plated a second positive plate from our O/Ns and left it to grow