Alberta NINT/31 July 2008

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(New page: < Back to notebook < Previous entry lab work (JD): Counted colonies on both mine and Shannon's plates from ...)
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                               -my plate had 100s on the negative control so I assumed the vector was not cut properly
                               -my plate had 100s on the negative control so I assumed the vector was not cut properly
               Conclusion: Re-digest K102011 with more enzyme, for 2 hours and run very slowly on gel
               Conclusion: Re-digest K102011 with more enzyme, for 2 hours and run very slowly on gel
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Revision as of 18:16, 6 August 2008

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lab work (JD): 

             Counted colonies on both mine and Shannon's plates from yesterday's ligations
                              -unfortunately equal numbers on negative plates and ligation plates so ligation did not work
                              -my plate had 100s on the negative control so I assumed the vector was not cut properly
             Conclusion: Re-digest K102011 with more enzyme, for 2 hours and run very slowly on gel

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