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Alberta NINT/8 July 2008
From 2008.igem.org
(Difference between revisions)
| Line 6: | Line 6: | ||
Transformation results: | Transformation results: | ||
ara + ara - | ara + ara - | ||
| + | K102022 ++++ ++++ | ||
| + | K102032 +++ +++ | ||
| + | K102032 + K102011 - ++ | ||
| + | K102032 + K102034 ++ ++ | ||
| + | K102032 + K102035 - + | ||
| + | K102032 + K102036 + + | ||
| + | where ++++ is the most blue and + is the least blue. - indicates white colonies. | ||
lab work (JD): | lab work (JD): | ||
Revision as of 20:40, 16 July 2008
lab work (SD):
Transformation results:
ara + ara -
K102022 ++++ ++++
K102032 +++ +++
K102032 + K102011 - ++
K102032 + K102034 ++ ++
K102032 + K102035 - +
K102032 + K102036 + +
where ++++ is the most blue and + is the least blue. - indicates white colonies.
lab work (JD):
Collected new quantitative data of K102003/E+P, K102006/E+P and newly cut pSB4A5
Carried out ligation of K102003+pSB4A5 and K102006+pSB4A5
Collected new pSB4A5 from iGEM binder
Transformed XL1-B cells with ligations
Transformed DB3.1 cells with new pSB4A5 part
Plated cells on LB +Amp50 plates
