Assembly Related Protocols

From 2008.igem.org

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==Newest EcoRI/BamHI Transfer method (7/31/08)==
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Digestion:
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*0.5ul  plasmid in entry vector
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*0.5ul  EcoRI
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*0.5ul  BamHI
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*1ul NEB2 + ATP buffer
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*7.5ul of water
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*incubate the digestion for 1hr. in the thermocycler, and 20 min for heat kill.
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No purification step.
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Ligation:
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*1ul of dilute assembly vector (or 0.5ul if undiluted)
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*0.5ul ligase
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Transform. (same as usual)
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Latest revision as of 15:41, 28 March 2016

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