Brown: Team Resistance/25 October 2008

25 October 2008

Conductance/Lysis Re-test

Same method as oct 22 but used 50 mL of same culture as control and test

For some reason, the conductivity of the control solution is always greater than that of the test solution. Most likely due to calibration of the two meters

At T=180min, added 600ul more arabinose solution, to make culture with 2% arabinose 2% 50mL solution = 1.04 mL At T=205, added 1mL more arabinose to actually make it 2% solution

Cells did not lyse today. Perhaps wrong plasmid used. Will make culture of s different glycerol stock for test tomorrow.

For purposes of current method, 50mL of culture is not needed for each test.

Will test 15mL of each culture tomorrow (total 30mL)and perhaps arabinose induction will be more efficient.

pVJ4 biobrick

concentration miniprep: 63.6ng/ul

Ran gel of part:

• Single band between 3 and 4kb

EcoRI/SpeI digest

• Used Buffer B due to potential star activity with EcoRI

Conductance/Lysis Re-test

• Same method as oct 22 but used 50 mL of same culture as control and test

• Time control Test

         OD	  OD
  *0	0.064	0.063
  *15	0.061	0.061
  *30	0.072	0.065
  *45	0.074	0.072
  *60	0.077	0.074
  *75	0.081	0.078
  *90	0.085	0.084
  *105	0.089	0.088
  *120	0.093	0.086
  *135	0.093	0.092
  *150	0.088	0.087
  *165	0.086	0.09
  *180	0.092	0.09
  *195	0.089	0.083
  *210	0.091	0.089
  *225	0.095	0.088
  *240	0.089	0.089
  *255	0.09	0.083
  *270	0.087	0.086

• For some reason, the conductivity of the control solution is always greater than that of the test solution. Most likely due to calibration of the two meters