Brown: Team Resistance/25 September 2008

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  • Growth seen in 3:1 and 6:1 ligations!
  • Ligation cultures miniprepped.
  • 3:1 Restriction Digest (X2)
  1. 1 ul EcoRI
  2. 1 ul HindIII
  3. 5 ul Buffer E
  4. .5 ul BSA
  5. 10 ul DNA
  6. 32.5 ul sterilized water
  • 6:1 Restriction Digest (X2)
  1. 1 ul EcoRI
  2. 1 ul HindIII
  3. 5 ul Buffer E
  4. .5 ul BSA
  5. 9 ul DNA
  6. 33.5 ul sterilized water
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