Brown: Team Resistance/9 July 2008

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  • OPTICAL DENSITY TEST:
  • We made multiple solutions of pVJ4 and added varying amounts of Arabinose to determine how long complete lysis takes. We also added Chloramphenicol (1:1 ratio) in addition to Arabinose to see whether it would speed up lysis.
  • Initial OD 50mL culture: .038

.2%(1) .2%(2) .4%(1) .4%(2) .2% + Chloramphenicol(1) .2% + Chloramphenicol(2) Control 3:45 PM .084 .085 .079 .079 .062 .053 .073 4:45 PM .065 .069 .040 .048 .044 .047 .076 5:55 PM .018 .018 .023 .021 .034 .050 .075 6:50 PM .014 .015 .013 .013 .050 .044 .071


  • The first few readings indicated an increase in the optical density. This is to be expected because the bacteria are in the mid-log phase and still growing. Growth must occur in order for the S, R, and Rz genes to be expressed and translated. Cell lysis is complete in four hours.
  • The addition of Chloramphenicol did not alter the rate of lysis.
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