Cellular Communication Systems

From 2008.igem.org

Revision as of 14:02, 29 October 2008 by Rcool (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Lux cell signaling system

Responsible: Andrew Gordon and Pallavi Penumetcha

Signal molecule: N-acyl-homoserine lactone (AHL) Generic term for a variety of species specific hormone-like molecules

Bacterial species: discovered in Vibrio fischeri known to work in E. coli

Receiver protein: LuxR protein receives signal from AHL; also has some control over transciption of luciferase

Signal molecule synthase: LuxI; also has some control over transciption of luciferase

Additional Information: "Quorum Quenching" aiiA (intracellular) lactonase reduces AHL concentration

800px-Luxrreceiverschematic.png

Resources

Lux Operon Pathway

AHL signaling molecules by species; some are specific to gram pos but may affect gram negs

Reducing Crosstalk in Lux System

References

Quorum Quenching to control Lux Pathway

[1] A Synthetic multicellular system for programmed pattern formation

Parts Needed:

LuxR + pro/term

RBS

LuxI + pro/term

LuxI sender

Retrieved from "http://2008.igem.org/Cellular_Communication_Systems"