Chiba/Sender experiments

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Project Design ( Sender 実験 | レシーバー実験 ) Making time delay | Our Goal


Quorum-Sensing Cross-talk

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Method

To characterize quorum sensing crosstalk, constitutive AHL senders were mixed with constitutive receivers and measure fluorescence intensity.

  1. Transformed Senders into E.coli strains(JW1908/XL10GOLD) and Receiver into E.coli strain(JW1908).
  2. Inoculated them independently in liquid media. Incubated at 37℃ 12h
  3. Mixed them.
  4. Incubated at 37℃ or 30℃.
  5. Measured intensity of green fluorescence at regular time intervals.

Result

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