"
Cutting a Specific Band from Agarose Gel
From 2008.igem.org
(Difference between revisions)
Riachalder (Talk | contribs) |
|||
| (One intermediate revision not shown) | |||
| Line 1: | Line 1: | ||
| - | [[Image:UVgel.jpg | + | {{:Team:Newcastle University/Header}} |
| + | {{:Team:Newcastle University/Template:UnderTheProtocol|page-title=[[Cutting a Specific Band from Agarose Gel]]}} | ||
| + | |||
| + | [[Image:UVgel.jpg|300px]] | ||
| + | |||
| + | A gel in the UV light box prior to cutting | ||
[[Image:jess_gel.jpg|thumb|right|250px|Jess cutting out a fragment from the gel using a sterile scalpel blade]] | [[Image:jess_gel.jpg|thumb|right|250px|Jess cutting out a fragment from the gel using a sterile scalpel blade]] | ||
| Line 11: | Line 16: | ||
* Using a sterilised scalpel blade, long wavelength(365 nm) and minimal exposure time, cut out the required band, including as little of the surrounding gel as possible. | * Using a sterilised scalpel blade, long wavelength(365 nm) and minimal exposure time, cut out the required band, including as little of the surrounding gel as possible. | ||
| - | * Place agarose gel band into a DNase-free 1.5 ml microcentrifuge tube. | + | * Place agarose gel band into a DNase-free 1.5 ml microcentrifuge tube. |
| + | |||
| + | * Weigh the microcentrifuge tube plus agarose band and calculate the weight of the actual agarose slice. | ||
* Proceed to [[Purifying DNA from Gel Slices]]. | * Proceed to [[Purifying DNA from Gel Slices]]. | ||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
Latest revision as of 16:03, 28 October 2008
Newcastle University
GOLD MEDAL WINNER 2008
| Home | Team | Original Aims | Software | Modelling | Proof of Concept Brick | Wet Lab | Conclusions |
|---|
Home >> Wet Lab >> Protocols >> Cutting a Specific Band from Agarose Gel
A gel in the UV light box prior to cutting
- After running the restricted plasmid on agarose gel (prefereably with a wide comb to increase well size), identify the specific band to be cut out.
- Weigh a DNase-free 1.5 ml microcentrifuge tube and record the weight.
- Place the gel in a UV light box (N.B. wear gloves and a UV filter mask and ensure sleeves are completely covering wrists to avoid direct UV light exposure).
- Using a sterilised scalpel blade, long wavelength(365 nm) and minimal exposure time, cut out the required band, including as little of the surrounding gel as possible.
- Place agarose gel band into a DNase-free 1.5 ml microcentrifuge tube.
- Weigh the microcentrifuge tube plus agarose band and calculate the weight of the actual agarose slice.
- Proceed to Purifying DNA from Gel Slices.
