Cutting a Specific Band from Agarose Gel
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[[Image:jess_gel.jpg|thumb|right|250px|Jess cutting out a fragment from the gel using a sterile scalpel blade]] | [[Image:jess_gel.jpg|thumb|right|250px|Jess cutting out a fragment from the gel using a sterile scalpel blade]] | ||
- | * After running the restricted plasmid on | + | * After running the restricted plasmid on agarose gel (prefereably with a wide comb to increase well size), identify the specific band to be cut out. |
- | * | + | |
- | Place the gel | + | * Weigh a DNase-free 1.5 ml microcentrifuge tube and record the weight. |
- | * Using a scalpel blade | + | |
- | * Place | + | * Place the gel in a UV light box (N.B. wear gloves and a UV filter mask and ensure sleeves are completely covering wrists to avoid direct UV light exposure). |
- | + | ||
+ | * Using a sterilised scalpel blade, long wavelength(365 nm) and minimal exposure time, cut out the required band, including as little of the surrounding gel as possible. | ||
+ | |||
+ | * Place agarose gel band into a DNase-free 1.5 ml microcentrifuge tube. | ||
+ | |||
* Proceed to [[Purifying DNA from Gel Slices]]. | * Proceed to [[Purifying DNA from Gel Slices]]. | ||
Revision as of 11:56, 19 September 2008
- After running the restricted plasmid on agarose gel (prefereably with a wide comb to increase well size), identify the specific band to be cut out.
- Weigh a DNase-free 1.5 ml microcentrifuge tube and record the weight.
- Place the gel in a UV light box (N.B. wear gloves and a UV filter mask and ensure sleeves are completely covering wrists to avoid direct UV light exposure).
- Using a sterilised scalpel blade, long wavelength(365 nm) and minimal exposure time, cut out the required band, including as little of the surrounding gel as possible.
- Place agarose gel band into a DNase-free 1.5 ml microcentrifuge tube.
- Proceed to Purifying DNA from Gel Slices.
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