Cutting a Specific Band from Agarose Gel

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Revision as of 15:18, 16 September 2008 by Riachalder (Talk | contribs)
A gel in the UV light box prior to cutting
Jess cutting out a fragment from the gel using a sterile scalpel blade
  • After running the restricted plasmid on an agarose gel (prefereably large-welled with as much plasmid as possible), identify the specific band to be cut out.
  • Record the weight of an Eppendorf tube.

Place the gel on a UV light box (N.B. wear gloves and a UV filter mask and ensure sleeves are completely covering wrists to avoid direct UV light exposure).

  • Using a scalpel blade that has been cleaned with ethanol, carefully cut out the band. Try to include as little of the surrounding gel as possible.
  • Place the gel slice in the Eppendorf tube.
  • Record the weight the Eppendorf tube and gel slice and work out the difference between this and the empty tube. This information is important for determining how much buffer to use for purifying the DNA from the gel.
  • Proceed to Purifying DNA from Gel Slices.
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