Cutting a Specific Band from Agarose Gel
Revision as of 16:03, 28 October 2008 by Mtaschuk
GOLD MEDAL WINNER 2008
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A gel in the UV light box prior to cutting
- After running the restricted plasmid on agarose gel (prefereably with a wide comb to increase well size), identify the specific band to be cut out.
- Weigh a DNase-free 1.5 ml microcentrifuge tube and record the weight.
- Place the gel in a UV light box (N.B. wear gloves and a UV filter mask and ensure sleeves are completely covering wrists to avoid direct UV light exposure).
- Using a sterilised scalpel blade, long wavelength(365 nm) and minimal exposure time, cut out the required band, including as little of the surrounding gel as possible.
- Place agarose gel band into a DNase-free 1.5 ml microcentrifuge tube.
- Weigh the microcentrifuge tube plus agarose band and calculate the weight of the actual agarose slice.
- Proceed to Purifying DNA from Gel Slices.