Cutting a Specific Band from Agarose Gel


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A gel in the UV light box prior to cutting

Jess cutting out a fragment from the gel using a sterile scalpel blade
  • After running the restricted plasmid on agarose gel (prefereably with a wide comb to increase well size), identify the specific band to be cut out.
  • Weigh a DNase-free 1.5 ml microcentrifuge tube and record the weight.
  • Place the gel in a UV light box (N.B. wear gloves and a UV filter mask and ensure sleeves are completely covering wrists to avoid direct UV light exposure).
  • Using a sterilised scalpel blade, long wavelength(365 nm) and minimal exposure time, cut out the required band, including as little of the surrounding gel as possible.
  • Place agarose gel band into a DNase-free 1.5 ml microcentrifuge tube.
  • Weigh the microcentrifuge tube plus agarose band and calculate the weight of the actual agarose slice.