Edinburgh/13 July 2008

From 2008.igem.org

Revision as of 14:07, 28 August 2008 by Andhi (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
< Previous Entry

Week 4

Sunday 13 July 08

  • Signs of growth on Cellulomonas plates 28 and 29.
  • Minipreps M25 to M30 of possible rbs+dxs clones from plate 34.
  • PCR P12 to obtain rbs+dxs fusion product in case minipreps didn't work. Template was ligation L11, and primers were rbs2clonf1 and pSB1A2insr1. Annealing was 58°C, extension 40s using KOD.
  • M25 to M30 were digested with SacI/SpeI and run on gel 12 along with undigested P12. This shows that M25 to M30 are no good.


Next Entry >