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Edinburgh/13 July 2008
From 2008.igem.org
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Week 4
Sunday 13 July 08
- Signs of growth on Cellulomonas plates 28 and 29.
- Minipreps M25 to M30 of possible rbs+dxs clones from plate 34.
- PCR P12 to obtain rbs+dxs fusion product in case minipreps didn't work. Template was ligation L11, and primers were rbs2clonf1 and pSB1A2insr1. Annealing was 58°C, extension 40s using KOD.
- M25 to M30 were digested with SacI/SpeI and run on gel 12 along with undigested P12. This shows that M25 to M30 are no good.
