Edinburgh/1 August 2008

From 2008.igem.org

(Difference between revisions)
(New page: <html> <head> <title>Edinburgh iGEM 2008</title> <script type="text/javascript"> //Drop Down Tabs Menu- Author: Dynamic Drive (http://www.dynamicdrive.com) //Created: May 16th, 07' var ta...)
 
(3 intermediate revisions not shown)
Line 1: Line 1:
-
<html>
+
<div id="header">{{Template:Team:Edinburgh/Templates/notebook-entry/header}}</div>
-
<head>
+
-
<title>Edinburgh iGEM 2008</title>
+
-
<script type="text/javascript">
+
-
//Drop Down Tabs Menu- Author: Dynamic Drive (http://www.dynamicdrive.com)
+
-
//Created: May 16th, 07'
+
-
var tabdropdown={
+
:::: '''[[Edinburgh/31_July_2008|< Previous Entry]]'''
-
disappeardelay: 200, //set delay in miliseconds before menu disappears onmouseout
+
-
disablemenuclick: false, //when user clicks on a menu item with a drop down menu, disable menu item's link?
+
-
enableiframeshim: 1, //1 or 0, for true or false
+
-
//No need to edit beyond here////////////////////////
+
== Week 7 ==
-
dropmenuobj: null, ie: document.all, firefox: document.getElementById&&!document.all, previousmenuitem:null,
+
=== Friday 1 August 08 ===
-
currentpageurl: window.location.href.replace("http://"+window.location.hostname, "").replace(/^\//, ""), //get current page url (minus hostname, ie: http://www.dynamicdrive.com/)
+
-
 
+
-
getposOffset:function(what, offsettype){
+
-
var totaloffset=(offsettype=="left")? what.offsetLeft : what.offsetTop;
+
-
var parentEl=what.offsetParent;
+
-
while (parentEl!=null){
+
-
totaloffset=(offsettype=="left")? totaloffset+parentEl.offsetLeft : totaloffset+parentEl.offsetTop;
+
-
parentEl=parentEl.offsetParent;
+
-
}
+
-
return totaloffset;
+
-
},
+
-
 
+
-
showhide:function(obj, e, obj2){ //obj refers to drop down menu, obj2 refers to tab menu item mouse is currently over
+
-
if (this.ie || this.firefox)
+
-
this.dropmenuobj.style.left=this.dropmenuobj.style.top="-500px"
+
-
if (e.type=="click" && obj.visibility==hidden || e.type=="mouseover"){
+
-
if (obj2.parentNode.className.indexOf("default")==-1) //if tab isn't a default selected one
+
-
obj2.parentNode.className="selected"
+
-
obj.visibility="visible"
+
-
}
+
-
else if (e.type=="click")
+
-
obj.visibility="hidden"
+
-
},
+
-
 
+
-
iecompattest:function(){
+
-
return (document.compatMode && document.compatMode!="BackCompat")? document.documentElement : document.body
+
-
},
+
-
 
+
-
clearbrowseredge:function(obj, whichedge){
+
-
var edgeoffset=0
+
-
if (whichedge=="rightedge"){
+
-
var windowedge=this.ie && !window.opera? this.standardbody.scrollLeft+this.standardbody.clientWidth-15 : window.pageXOffset+window.innerWidth-15
+
-
this.dropmenuobj.contentmeasure=this.dropmenuobj.offsetWidth
+
-
if (windowedge-this.dropmenuobj.x < this.dropmenuobj.contentmeasure)  //move menu to the left?
+
-
edgeoffset=this.dropmenuobj.contentmeasure-obj.offsetWidth
+
-
}
+
-
else{
+
-
var topedge=this.ie && !window.opera? this.standardbody.scrollTop : window.pageYOffset
+
-
var windowedge=this.ie && !window.opera? this.standardbody.scrollTop+this.standardbody.clientHeight-15 : window.pageYOffset+window.innerHeight-18
+
-
this.dropmenuobj.contentmeasure=this.dropmenuobj.offsetHeight
+
-
if (windowedge-this.dropmenuobj.y < this.dropmenuobj.contentmeasure){ //move up?
+
-
edgeoffset=this.dropmenuobj.contentmeasure+obj.offsetHeight
+
-
if ((this.dropmenuobj.y-topedge)<this.dropmenuobj.contentmeasure) //up no good either?
+
-
edgeoffset=this.dropmenuobj.y+obj.offsetHeight-topedge
+
-
}
+
-
this.dropmenuobj.firstlink.style.borderTopWidth=(edgeoffset==0)? 0 : "1px" //Add 1px top border to menu if dropping up
+
-
}
+
-
return edgeoffset
+
-
},
+
-
 
+
-
dropit:function(obj, e, dropmenuID){
+
-
if (this.dropmenuobj!=null){ //hide previous menu
+
-
this.dropmenuobj.style.visibility="hidden" //hide menu
+
-
if (this.previousmenuitem!=null && this.previousmenuitem!=obj){
+
-
if (this.previousmenuitem.parentNode.className.indexOf("default")==-1) //If the tab isn't a default selected one
+
-
this.previousmenuitem.parentNode.className=""
+
-
}
+
-
}
+
-
this.clearhidemenu()
+
-
if (this.ie||this.firefox){
+
-
obj.onmouseout=function(){tabdropdown.delayhidemenu(obj)}
+
-
obj.onclick=function(){return !tabdropdown.disablemenuclick} //disable main menu item link onclick?
+
-
this.dropmenuobj=document.getElementById(dropmenuID)
+
-
this.dropmenuobj.onmouseover=function(){tabdropdown.clearhidemenu()}
+
-
this.dropmenuobj.onmouseout=function(e){tabdropdown.dynamichide(e, obj)}
+
-
this.dropmenuobj.onclick=function(){tabdropdown.delayhidemenu(obj)}
+
-
this.showhide(this.dropmenuobj.style, e, obj)
+
-
this.dropmenuobj.x=this.getposOffset(obj, "left")
+
-
this.dropmenuobj.y=this.getposOffset(obj, "top")
+
-
this.dropmenuobj.style.left=this.dropmenuobj.x-this.clearbrowseredge(obj, "rightedge")+"px"
+
-
this.dropmenuobj.style.top=this.dropmenuobj.y-this.clearbrowseredge(obj, "bottomedge")+obj.offsetHeight+1+"px"
+
-
this.previousmenuitem=obj //remember main menu item mouse moved out from (and into current menu item)
+
-
this.positionshim() //call iframe shim function
+
-
}
+
-
},
+
-
 
+
-
contains_firefox:function(a, b) {
+
-
while (b.parentNode)
+
-
if ((b = b.parentNode) == a)
+
-
return true;
+
-
return false;
+
-
},
+
-
 
+
-
dynamichide:function(e, obj2){ //obj2 refers to tab menu item mouse is currently over
+
-
var evtobj=window.event? window.event : e
+
-
if (this.ie&&!this.dropmenuobj.contains(evtobj.toElement))
+
-
this.delayhidemenu(obj2)
+
-
else if (this.firefox&&e.currentTarget!= evtobj.relatedTarget&& !this.contains_firefox(evtobj.currentTarget, evtobj.relatedTarget))
+
-
this.delayhidemenu(obj2)
+
-
},
+
-
 
+
-
delayhidemenu:function(obj2){
+
-
this.delayhide=setTimeout(function(){tabdropdown.dropmenuobj.style.visibility='hidden'; if (obj2.parentNode.className.indexOf('default')==-1) obj2.parentNode.className=''},this.disappeardelay) //hide menu
+
-
},
+
-
 
+
-
clearhidemenu:function(){
+
-
if (this.delayhide!="undefined")
+
-
clearTimeout(this.delayhide)
+
-
},
+
-
 
+
-
positionshim:function(){ //display iframe shim function
+
-
if (this.enableiframeshim && typeof this.shimobject!="undefined"){
+
-
if (this.dropmenuobj.style.visibility=="visible"){
+
-
this.shimobject.style.width=this.dropmenuobj.offsetWidth+"px"
+
-
this.shimobject.style.height=this.dropmenuobj.offsetHeight+"px"
+
-
this.shimobject.style.left=this.dropmenuobj.style.left
+
-
this.shimobject.style.top=this.dropmenuobj.style.top
+
-
}
+
-
this.shimobject.style.display=(this.dropmenuobj.style.visibility=="visible")? "block" : "none"
+
-
}
+
-
},
+
-
 
+
-
hideshim:function(){
+
-
if (this.enableiframeshim && typeof this.shimobject!="undefined")
+
-
this.shimobject.style.display='none'
+
-
},
+
-
 
+
-
isSelected:function(menuurl){
+
-
var menuurl=menuurl.replace("http://"+menuurl.hostname, "").replace(/^\//, "")
+
-
return (tabdropdown.currentpageurl==menuurl)
+
-
},
+
-
 
+
-
init:function(menuid, dselected){
+
-
this.standardbody=(document.compatMode=="CSS1Compat")? document.documentElement : document.body //create reference to common "body" across doctypes
+
-
var menuitems=document.getElementById(menuid).getElementsByTagName("a")
+
-
for (var i=0; i<menuitems.length; i++){
+
-
if (menuitems[i].getAttribute("rel")){
+
-
var relvalue=menuitems[i].getAttribute("rel")
+
-
document.getElementById(relvalue).firstlink=document.getElementById(relvalue).getElementsByTagName("a")[0]
+
-
menuitems[i].onmouseover=function(e){
+
-
var event=typeof e!="undefined"? e : window.event
+
-
tabdropdown.dropit(this, event, this.getAttribute("rel"))
+
-
}
+
-
}
+
-
if (dselected=="auto" && typeof setalready=="undefined" && this.isSelected(menuitems[i].href)){
+
-
menuitems[i].parentNode.className+=" selected default"
+
-
var setalready=true
+
-
}
+
-
else if (parseInt(dselected)==i)
+
-
menuitems[i].parentNode.className+=" selected default"
+
-
}
+
-
}
+
-
 
+
-
}
+
-
</script>
+
-
<style>
+
-
.ddcolortabs{
+
-
padding: 0;
+
-
width: 100%;
+
-
background: transparent;
+
-
voice-family: "\"}\"";
+
-
voice-family: inherit;
+
-
}
+
-
 
+
-
.ddcolortabs ul{
+
-
font: normal 11px Arial, Verdana, sans-serif;
+
-
margin:0;
+
-
padding:0;
+
-
list-style:none;
+
-
}
+
-
 
+
-
.ddcolortabs li{
+
-
display:inline;
+
-
margin:0 2px 0 0;
+
-
padding:0;
+
-
text-transform:uppercase;
+
-
}
+
-
 
+
-
 
+
-
.ddcolortabs a{
+
-
float:left;
+
-
color: white;
+
-
background: black url(media/color_tabs_left.gif) no-repeat left top;
+
-
margin:0 2px 0 0;
+
-
padding:0 0 1px 3px;
+
-
text-decoration:none;
+
-
letter-spacing: 1px;
+
-
}
+
-
 
+
-
.ddcolortabs a span{
+
-
float:left;
+
-
display:block;
+
-
background: transparent url(media/color_tabs_right.gif) no-repeat right top;
+
-
padding: 4px 8px 2px 7px;
+
-
}
+
-
 
+
-
.ddcolortabs a span{
+
-
float:none;
+
-
}
+
-
 
+
-
.ddcolortabs a:hover{
+
-
background-color: #591f20;
+
-
}
+
-
 
+
-
.ddcolortabs a:hover span{
+
-
background-color: #591f20;
+
-
}
+
-
 
+
-
.ddcolortabs .selected a, #ddcolortabs .selected a span{ /*currently selected tab*/
+
-
background-color: #591f20;
+
-
}
+
-
 
+
-
.ddcolortabsline{
+
-
clear: both;
+
-
padding: 0;
+
-
width: 100%;
+
-
height: 8px;
+
-
line-height: 8px;
+
-
background: black;
+
-
border-top: 1px solid #fff; /*Remove this to remove border between bar and tabs*/
+
-
}
+
-
 
+
-
/* ######### Style for Drop Down Menu ######### */
+
-
 
+
-
.dropmenudiv_a{
+
-
position:absolute;
+
-
top: 0;
+
-
border: 1px solid black; /*THEME CHANGE HERE*/
+
-
border-top-width: 8px; /*Top border width. Should match height of .ddcolortabsline above*/
+
-
border-bottom-width: 0;
+
-
font:normal 12px Arial;
+
-
line-height:18px;
+
-
z-index:100;
+
-
background-color: white;
+
-
width: 200px;
+
-
visibility: hidden;
+
-
}
+
-
 
+
-
 
+
-
.dropmenudiv_a a{
+
-
width: auto;
+
-
display: block;
+
-
text-indent: 5px;
+
-
border-top: 0 solid #678b3f;
+
-
border-bottom: 1px solid #678b3f; /*THEME CHANGE HERE*/
+
-
padding: 2px 0;
+
-
text-decoration: none;
+
-
color: black;
+
-
}
+
-
 
+
-
* html .dropmenudiv_a a{ /*IE only hack*/
+
-
width: 100%;
+
-
}
+
-
 
+
-
.dropmenudiv_a a:hover{ /*THEME CHANGE HERE*/
+
-
background-color: #8a3c3d;
+
-
color: white;
+
-
}
+
-
</style>
+
-
</head>
+
-
 
+
-
<!-- CSS for Drop Down Tabs Menu #1 -->
+
-
<link rel="stylesheet" type="text/css" href="ddcolortabs.css" />
+
-
 
+
-
<div id="colortab" class="ddcolortabs">
+
-
<ul>
+
-
<li><a href="https://2008.igem.org/Team:Edinburgh" title="Home"><span>Home</span></a></li>
+
-
<li><a href="https://2008.igem.org/Team:Edinburgh/Project" title="Project" rel="dropmenu1_a"><span>The Project</span></a></li>
+
-
<li><a href="https://2008.igem.org/Team:Edinburgh/Team" title="Team" ><span>The Team</span></a></li>
+
-
<li><a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2008&group=Edinburgh" title="Project" rel="dropmenu1_a"><span>BioBrick Parts</span></a></li>
+
-
<li><a href="https://2008.igem.org/Team:Edinburgh/Modeling" title="Modelling" rel="dropmenu2_a"><span>Modelling</span></a></li>
+
-
<li><a href="https://2008.igem.org/Team:Edinburgh/Notebook" title="Notebook"><span>Notebook</span></a></li>
+
-
</ul>
+
-
</div>
+
-
<div class="ddcolortabsline">&nbsp;</div>
+
-
<div id="dropmenu1_a" class="dropmenudiv_a">
+
-
<a href="https://2008.igem.org/Team:Edinburgh/Team">Overview</a>
+
-
<a href="https://2008.igem.org/Team:Edinburgh/Team">Step1</a>
+
-
<a href="https://2008.igem.org/Team:Edinburgh/Team">Step2</a>
+
-
</div>
+
-
<script type="text/javascript">
+
-
//SYNTAX: tabdropdown.init("menu_id", [integer OR "auto"])
+
-
tabdropdown.init("colortab", 3)
+
-
</script>
+
-
</html>
+
-
 
+
-
=== Week 7 ===
+
-
==== Friday 1 August 08 ====
+
* Primers for pCstA ordered. (CF)
* Primers for pCstA ordered. (CF)
-
* White colonies from Thursday's transformations were subplated as follows...
+
* White colonies from Thursday's transformations were subplated as follows:
** Plate 76 (L26: pSB1A2+rbs+''appY'') to '''Plate 83'''
** Plate 76 (L26: pSB1A2+rbs+''appY'') to '''Plate 83'''
** Plate 77 (L27: pSB1A2+rbs+''crtB'') to '''Plate 84'''
** Plate 77 (L27: pSB1A2+rbs+''crtB'') to '''Plate 84'''
** Plate 80 (L28: pSB1A2+''cenA'') to '''Plate 85'''
** Plate 80 (L28: pSB1A2+''cenA'') to '''Plate 85'''
** Plates 81/82 (L29: pSB1A2+''cex'') to '''Plate 86'''. (AM/HX/OG)
** Plates 81/82 (L29: pSB1A2+''cex'') to '''Plate 86'''. (AM/HX/OG)
-
* ''First digestion'': M68 to M71 (pSB1A2+''glgC''-mut1,2) with EcoRI/PstI, M72 to M75 (pSB1A2+rbs+''dxs'') with EcoRI, M76 to M79 (BABEL2+''crtE'') with EcoRI/PstI. Run on '''Gel 33'''. Results: M68, 69, 71: vector band around 2 kb, no ''glgC'' band; M72, 73, 74: single band around 4 kb, expected length for vector+gene; M76-79: ''crtE'' band <1 kb, vector band ~2 kb. It is not clear into which vector ''crtE'' (M76~79) is inserted, so sequencing will be necessary.
+
* ''First digestion'': M68-M71 (pSB1A2+''glgC''-mut1,2) with EcoRI/PstI, M72-M75 (pSB1A2+rbs+''dxs'') with EcoRI, M76-M79 (BABEL2+''crtE'') with EcoRI/PstI. Run on '''Gel 33'''. Results: M68, 69, 71: vector band around 2 kb, no ''glgC'' band; M72, 73, 74: single band around 4 kb, expected length for vector+gene; M76-79: ''crtE'' band <1 kb, vector band ~2 kb. It is not clear into which vector ''crtE'' (M76-79) is inserted, so sequencing will be necessary. (AM)
-
* ''Second digestion'': M68 and M69 with a) EcoRI, b) EcoRI/PstI; M72 with a) EcoRI b) SacI/SpeI. Run on '''Gel 34'''. Results: Single and double digests of M68 and M69 yielded similar results, hence pSB1A2+''glgC''-mut1,2 ligation or transformation failed; digests of M72 yielded expected bands, hence pSB1A2+rbs+''dxs'' successful. (AM)
+
* ''Second digestion'': M68 and M69 with a) EcoRI, b) EcoRI/PstI; M72 with a) EcoRI b) SacI/SpeI. Run on '''Gel 34'''. Results: Single and double digests of M68 and M69 yielded similar results, hence pSB1A2+''glgC''-mut1,2 ligation or transformation failed; digests of M72 yielded expected bands, hence pSB1A2+rbs+''dxs'' probably successful. (AM)
-
* P36 (''glgC''-mut1,2 excision from BABEL2) and L25 (P36 + Edinbrick1) were run again on '''Gel 35'''. Results: P36 viewed at lower illumination yields one band of the desired length (1.5kb); L25 yields two bands around 1.5kb, a thick band at 3kb and several fainter bands including one at 2kb (length of pSB1A2). (AM)
+
* P36 (''glgC''-mut1,2 excision from BABEL2) and L25 (P36 + Edinbrick1) were run again on '''Gel 35'''. Results: P36 viewed at lower illumination yields one band of the desired length (1.5kb); L25 yields two bands around 1.5kb, a thick band at 3kb and several fainter bands including one at 2kb (length of pSB1A2). (AM)<br />
 +
<br />
 +
 
 +
:::: '''[[Edinburgh/3_August_2008|Next Entry >]]'''

Latest revision as of 14:13, 28 August 2008

< Previous Entry

Week 7

Friday 1 August 08

  • Primers for pCstA ordered. (CF)
  • White colonies from Thursday's transformations were subplated as follows:
    • Plate 76 (L26: pSB1A2+rbs+appY) to Plate 83
    • Plate 77 (L27: pSB1A2+rbs+crtB) to Plate 84
    • Plate 80 (L28: pSB1A2+cenA) to Plate 85
    • Plates 81/82 (L29: pSB1A2+cex) to Plate 86. (AM/HX/OG)
  • First digestion: M68-M71 (pSB1A2+glgC-mut1,2) with EcoRI/PstI, M72-M75 (pSB1A2+rbs+dxs) with EcoRI, M76-M79 (BABEL2+crtE) with EcoRI/PstI. Run on Gel 33. Results: M68, 69, 71: vector band around 2 kb, no glgC band; M72, 73, 74: single band around 4 kb, expected length for vector+gene; M76-79: crtE band <1 kb, vector band ~2 kb. It is not clear into which vector crtE (M76-79) is inserted, so sequencing will be necessary. (AM)
  • Second digestion: M68 and M69 with a) EcoRI, b) EcoRI/PstI; M72 with a) EcoRI b) SacI/SpeI. Run on Gel 34. Results: Single and double digests of M68 and M69 yielded similar results, hence pSB1A2+glgC-mut1,2 ligation or transformation failed; digests of M72 yielded expected bands, hence pSB1A2+rbs+dxs probably successful. (AM)
  • P36 (glgC-mut1,2 excision from BABEL2) and L25 (P36 + Edinbrick1) were run again on Gel 35. Results: P36 viewed at lower illumination yields one band of the desired length (1.5kb); L25 yields two bands around 1.5kb, a thick band at 3kb and several fainter bands including one at 2kb (length of pSB1A2). (AM)


Next Entry >
Retrieved from "http://2008.igem.org/Edinburgh/1_August_2008"