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Edinburgh/Notebook/Ligation products
From 2008.igem.org
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'''L10:''' Ligation of Edinbrick1 with ''crtI'' (mutant lacking PstI sites, I hope). (10.07.08)<br /> | '''L10:''' Ligation of Edinbrick1 with ''crtI'' (mutant lacking PstI sites, I hope). (10.07.08)<br /> | ||
'''L11:''' repeat ligation of synthetic ribosome binding site to M2 (pSB1A2+''dxs'', BBa_K118000). (11.07.08: Yan, HX)<br /> | '''L11:''' repeat ligation of synthetic ribosome binding site to M2 (pSB1A2+''dxs'', BBa_K118000). (11.07.08: Yan, HX)<br /> | ||
| - | '''L12:''' rbs ligated with ''appY''.<br /> | + | '''L12:''' rbs ligated with pSB1A2+''appY'' (M15). (14.07.08)<br /> |
| - | '''L13 and L14:''' self ligations of ''glgC'' | + | '''L13 and L14:''' self ligations of P13 and P14 (BABEL2+''glgC''-mut1,2). (14.07.08)<br /> |
| - | '''L15:''' ligation of Edinbrick1 with rbs+''dxs'' fusion PCR product (P12). - Failed<br /> | + | '''L15:''' ligation of Edinbrick1 with rbs+''dxs'' fusion PCR product (P12). (14.07.08) - Failed<br /> |
'''L16:''' Ligation of Edinbrick1 with rbs+''appY'' fusion PCR product (P15) (17/07/08) - Failed<br /> | '''L16:''' Ligation of Edinbrick1 with rbs+''appY'' fusion PCR product (P15) (17/07/08) - Failed<br /> | ||
'''L17:''' Ligation of ''crtB'' (M36) to rbs (22/07/08)<br /> | '''L17:''' Ligation of ''crtB'' (M36) to rbs (22/07/08)<br /> | ||
Revision as of 16:33, 21 August 2008
L1: Edinbrick1+dxs (26.08.08)
L2: Edinbrick1+appY (26.08.08)
L3: Babel1+glgC (26.08.08)
L4: Babel2+glgC (26.08.08)
L5: Edinbrick1+appY repeat (initially labelled L4 in error) (04.07.08)
L6: Self ligation of mutagenic PCR P6 (BABEL2+glgC-mut1). (07.07.08)
L7: Self ligation of mutagenic PCR P7 (BABEL2+glgC-mut2). (07.07.08)
L8: Ligation of synthetic ribosome binding site to M2 (pSB1A2+dxs, BBa_K118000). (09.07.08)
L9: Ligation of Edinbrick1 with crtB. (10.07.08)
L10: Ligation of Edinbrick1 with crtI (mutant lacking PstI sites, I hope). (10.07.08)
L11: repeat ligation of synthetic ribosome binding site to M2 (pSB1A2+dxs, BBa_K118000). (11.07.08: Yan, HX)
L12: rbs ligated with pSB1A2+appY (M15). (14.07.08)
L13 and L14: self ligations of P13 and P14 (BABEL2+glgC-mut1,2). (14.07.08)
L15: ligation of Edinbrick1 with rbs+dxs fusion PCR product (P12). (14.07.08) - Failed
L16: Ligation of Edinbrick1 with rbs+appY fusion PCR product (P15) (17/07/08) - Failed
L17: Ligation of crtB (M36) to rbs (22/07/08)
L18: Ligation of crtI (M42) to rbs (22/07/08)
L19: pSB1A2+rbs+dxs ligation product (From P12) (25/07/08: Yan/OG)
L20: pSB1A2+rbs+appY ligation product (From P15) (25/07/08: Yan/OG)
L21: pSB1A2+rbs+crtB ligation product (From P28) (25/07/08: Yan/OG)
L22: pSB1A2+rbs+crtI ligation product (From P29) (25/07/08: Yan/OG)
L23: PzntA+pSB1A2 ligation product (From P31) (25/07/08: Yan/OG)
L24: Ligation of crtE to pSB1A2 (25/07/08: CF)
L25: Ligation of glgC double mutant (P36) to Edinbrick 1 (28.07.2008: AM)
L26: Ligation of Edi+P15 XP - rbs+appY (30.07.2008: HX)
L27: Ligation of Edi+P28 XP - rbs+crtB (30.07.2008: HX)
L28: Putative cenA ligated to Edinbrick1 (30.07.08: AM)
L29: Putative cex ligated to Edinbrick1 (30.07.08: AM)
L30: Self-ligation of P15 (3rd mutagenesis of glgC-mut1,2) (05.08.08: HX)
L31: Ligation of P36 (glgC-mut1,2, hopefully, could be P39) to Edinbrick1 (05.08.08: OG)
L32: Ligation of rbs+crtB (M67) to rbs+crtI (M50) to create crtB/crtI (07.08.08: AM, Yan)
L33: Ligation of PcstA (P54) to Edinbrick1 (08.08.08: OG)
