Edinburgh/Notebook/Ligation products

From 2008.igem.org

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<html>
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<div id="header">{{Template:Team:Edinburgh/Templates/Header}}</div>
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<head>
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<title>Edinburgh iGem 2008</title>
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<script type="text/javascript">
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//Drop Down Tabs Menu- Author: Dynamic Drive (http://www.dynamicdrive.com)
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//Created: May 16th, 07'
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var tabdropdown={
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'''[[Team:Edinburgh/Notebook|< Back to Notebook]]'''
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* disappeardelay: 200, //set delay in miliseconds before menu disappears onmouseout
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disablemenuclick: false, //when user clicks on a menu item with a drop down menu, disable menu item's link?
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enableiframeshim: 1, //1 or 0, for true or false
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//No need to edit beyond here////////////////////////
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== Ligation Products ==
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dropmenuobj: null, ie: document.all, firefox: document.getElementById&&!document.all, previousmenuitem:null,
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currentpageurl: window.location.href.replace("http://"+window.location.hostname, "").replace(/^\//, ""), //get current page url (minus hostname, ie: http://www.dynamicdrive.com/)
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getposOffset:function(what, offsettype){
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var totaloffset=(offsettype=="left")? what.offsetLeft : what.offsetTop;
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var parentEl=what.offsetParent;
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while (parentEl!=null){
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totaloffset=(offsettype=="left")? totaloffset+parentEl.offsetLeft : totaloffset+parentEl.offsetTop;
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parentEl=parentEl.offsetParent;
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}
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return totaloffset;
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},
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showhide:function(obj, e, obj2){ //obj refers to drop down menu, obj2 refers to tab menu item mouse is currently over
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if (this.ie || this.firefox)
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this.dropmenuobj.style.left=this.dropmenuobj.style.top="-500px"
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if (e.type=="click" && obj.visibility==hidden || e.type=="mouseover"){
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if (obj2.parentNode.className.indexOf("default")==-1) //if tab isn't a default selected one
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obj2.parentNode.className="selected"
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obj.visibility="visible"
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}
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else if (e.type=="click")
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obj.visibility="hidden"
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},
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iecompattest:function(){
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return (document.compatMode && document.compatMode!="BackCompat")? document.documentElement : document.body
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},
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clearbrowseredge:function(obj, whichedge){
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var edgeoffset=0
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if (whichedge=="rightedge"){
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var windowedge=this.ie && !window.opera? this.standardbody.scrollLeft+this.standardbody.clientWidth-15 : window.pageXOffset+window.innerWidth-15
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this.dropmenuobj.contentmeasure=this.dropmenuobj.offsetWidth
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if (windowedge-this.dropmenuobj.x < this.dropmenuobj.contentmeasure)  //move menu to the left?
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edgeoffset=this.dropmenuobj.contentmeasure-obj.offsetWidth
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}
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else{
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var topedge=this.ie && !window.opera? this.standardbody.scrollTop : window.pageYOffset
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-
var windowedge=this.ie && !window.opera? this.standardbody.scrollTop+this.standardbody.clientHeight-15 : window.pageYOffset+window.innerHeight-18
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this.dropmenuobj.contentmeasure=this.dropmenuobj.offsetHeight
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-
if (windowedge-this.dropmenuobj.y < this.dropmenuobj.contentmeasure){ //move up?
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edgeoffset=this.dropmenuobj.contentmeasure+obj.offsetHeight
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if ((this.dropmenuobj.y-topedge)<this.dropmenuobj.contentmeasure) //up no good either?
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edgeoffset=this.dropmenuobj.y+obj.offsetHeight-topedge
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}
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this.dropmenuobj.firstlink.style.borderTopWidth=(edgeoffset==0)? 0 : "1px" //Add 1px top border to menu if dropping up
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}
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return edgeoffset
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},
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dropit:function(obj, e, dropmenuID){
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if (this.dropmenuobj!=null){ //hide previous menu
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this.dropmenuobj.style.visibility="hidden" //hide menu
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if (this.previousmenuitem!=null && this.previousmenuitem!=obj){
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if (this.previousmenuitem.parentNode.className.indexOf("default")==-1) //If the tab isn't a default selected one
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this.previousmenuitem.parentNode.className=""
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}
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}
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this.clearhidemenu()
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if (this.ie||this.firefox){
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obj.onmouseout=function(){tabdropdown.delayhidemenu(obj)}
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obj.onclick=function(){return !tabdropdown.disablemenuclick} //disable main menu item link onclick?
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this.dropmenuobj=document.getElementById(dropmenuID)
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this.dropmenuobj.onmouseover=function(){tabdropdown.clearhidemenu()}
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this.dropmenuobj.onmouseout=function(e){tabdropdown.dynamichide(e, obj)}
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this.dropmenuobj.onclick=function(){tabdropdown.delayhidemenu(obj)}
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this.showhide(this.dropmenuobj.style, e, obj)
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this.dropmenuobj.x=this.getposOffset(obj, "left")
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this.dropmenuobj.y=this.getposOffset(obj, "top")
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this.dropmenuobj.style.left=this.dropmenuobj.x-this.clearbrowseredge(obj, "rightedge")+"px"
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this.dropmenuobj.style.top=this.dropmenuobj.y-this.clearbrowseredge(obj, "bottomedge")+obj.offsetHeight+1+"px"
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this.previousmenuitem=obj //remember main menu item mouse moved out from (and into current menu item)
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this.positionshim() //call iframe shim function
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}
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},
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contains_firefox:function(a, b) {
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while (b.parentNode)
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if ((b = b.parentNode) == a)
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return true;
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return false;
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},
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dynamichide:function(e, obj2){ //obj2 refers to tab menu item mouse is currently over
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var evtobj=window.event? window.event : e
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if (this.ie&&!this.dropmenuobj.contains(evtobj.toElement))
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this.delayhidemenu(obj2)
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else if (this.firefox&&e.currentTarget!= evtobj.relatedTarget&& !this.contains_firefox(evtobj.currentTarget, evtobj.relatedTarget))
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this.delayhidemenu(obj2)
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},
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delayhidemenu:function(obj2){
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this.delayhide=setTimeout(function(){tabdropdown.dropmenuobj.style.visibility='hidden'; if (obj2.parentNode.className.indexOf('default')==-1) obj2.parentNode.className=''},this.disappeardelay) //hide menu
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},
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clearhidemenu:function(){
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if (this.delayhide!="undefined")
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clearTimeout(this.delayhide)
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},
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positionshim:function(){ //display iframe shim function
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if (this.enableiframeshim && typeof this.shimobject!="undefined"){
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if (this.dropmenuobj.style.visibility=="visible"){
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this.shimobject.style.width=this.dropmenuobj.offsetWidth+"px"
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this.shimobject.style.height=this.dropmenuobj.offsetHeight+"px"
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this.shimobject.style.left=this.dropmenuobj.style.left
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this.shimobject.style.top=this.dropmenuobj.style.top
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}
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this.shimobject.style.display=(this.dropmenuobj.style.visibility=="visible")? "block" : "none"
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-
}
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},
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hideshim:function(){
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if (this.enableiframeshim && typeof this.shimobject!="undefined")
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this.shimobject.style.display='none'
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},
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isSelected:function(menuurl){
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var menuurl=menuurl.replace("http://"+menuurl.hostname, "").replace(/^\//, "")
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return (tabdropdown.currentpageurl==menuurl)
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},
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init:function(menuid, dselected){
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this.standardbody=(document.compatMode=="CSS1Compat")? document.documentElement : document.body //create reference to common "body" across doctypes
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var menuitems=document.getElementById(menuid).getElementsByTagName("a")
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-
for (var i=0; i<menuitems.length; i++){
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-
if (menuitems[i].getAttribute("rel")){
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var relvalue=menuitems[i].getAttribute("rel")
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-
document.getElementById(relvalue).firstlink=document.getElementById(relvalue).getElementsByTagName("a")[0]
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-
menuitems[i].onmouseover=function(e){
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var event=typeof e!="undefined"? e : window.event
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tabdropdown.dropit(this, event, this.getAttribute("rel"))
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-
}
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}
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if (dselected=="auto" && typeof setalready=="undefined" && this.isSelected(menuitems[i].href)){
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menuitems[i].parentNode.className+=" selected default"
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-
var setalready=true
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-
}
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-
else if (parseInt(dselected)==i)
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menuitems[i].parentNode.className+=" selected default"
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-
}
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-
}
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-
 
+
-
}
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</script>
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<style>
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-
.ddcolortabs{
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-
padding: 0;
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-
width: 100%;
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-
background: transparent;
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-
voice-family: "\"}\"";
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-
voice-family: inherit;
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-
}
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-
 
+
-
.ddcolortabs ul{
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font: normal 11px Arial, Verdana, sans-serif;
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-
margin:0;
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-
padding:0;
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-
list-style:none;
+
-
}
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-
 
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-
.ddcolortabs li{
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display:inline;
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-
margin:0 2px 0 0;
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-
padding:0;
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-
text-transform:uppercase;
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-
}
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-
 
+
-
 
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-
.ddcolortabs a{
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float:left;
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color: white;
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-
background: black url(media/color_tabs_left.gif) no-repeat left top;
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margin:0 2px 0 0;
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padding:0 0 1px 3px;
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text-decoration:none;
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-
letter-spacing: 1px;
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-
}
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-
 
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.ddcolortabs a span{
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float:left;
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display:block;
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-
background: transparent url(media/color_tabs_right.gif) no-repeat right top;
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-
padding: 4px 8px 2px 7px;
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-
}
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-
 
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-
.ddcolortabs a span{
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float:none;
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-
}
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.ddcolortabs a:hover{
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background-color: #591f20;
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-
}
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-
 
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-
.ddcolortabs a:hover span{
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-
background-color: #591f20;
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-
}
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.ddcolortabs .selected a, #ddcolortabs .selected a span{ /*currently selected tab*/
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-
background-color: #591f20;
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-
}
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-
 
+
-
.ddcolortabsline{
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-
clear: both;
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-
padding: 0;
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-
width: 100%;
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-
height: 8px;
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-
line-height: 8px;
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-
background: black;
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border-top: 1px solid #fff; /*Remove this to remove border between bar and tabs*/
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-
}
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-
 
+
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/* ######### Style for Drop Down Menu ######### */
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-
 
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.dropmenudiv_a{
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position:absolute;
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-
top: 0;
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-
border: 1px solid black; /*THEME CHANGE HERE*/
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-
border-top-width: 8px; /*Top border width. Should match height of .ddcolortabsline above*/
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-
border-bottom-width: 0;
+
-
font:normal 12px Arial;
+
-
line-height:18px;
+
-
z-index:100;
+
-
background-color: white;
+
-
width: 200px;
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-
visibility: hidden;
+
-
}
+
-
 
+
-
 
+
-
.dropmenudiv_a a{
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-
width: auto;
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-
display: block;
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-
text-indent: 5px;
+
-
border-top: 0 solid #678b3f;
+
-
border-bottom: 1px solid #678b3f; /*THEME CHANGE HERE*/
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-
padding: 2px 0;
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-
text-decoration: none;
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-
color: black;
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-
}
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-
 
+
-
* html .dropmenudiv_a a{ /*IE only hack*/
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-
width: 100%;
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-
}
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-
 
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-
.dropmenudiv_a a:hover{ /*THEME CHANGE HERE*/
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-
background-color: #8a3c3d;
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-
color: white;
+
-
}
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-
</style>
+
-
</head>
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-
 
+
-
<!-- CSS for Drop Down Tabs Menu #1 -->
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<link rel="stylesheet" type="text/css" href="ddcolortabs.css" />
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-
 
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<div id="colortab" class="ddcolortabs">
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<ul>
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<li><a href="https://2008.igem.org/Team:Edinburgh" title="Home"><span>Home</span></a></li>
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<li><a href="https://2008.igem.org/Team:Edinburgh/Project" title="Project" rel="dropmenu1_a"><span>The Project</span></a></li>
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<li><a href="https://2008.igem.org/Team:Edinburgh/Team" title="Team" ><span>The Team</span></a></li>
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<li><a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2008&group=Edinburgh" title="Project" rel="dropmenu1_a"><span>BioBrick Parts</span></a></li>
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<li><a href="https://2008.igem.org/Team:Edinburgh/Modeling" title="Modelling" rel="dropmenu2_a"><span>Modelling</span></a></li>
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<li><a href="https://2008.igem.org/Team:Edinburgh/Notebook" title="Notebook"><span>Notebook</span></a></li>
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</ul>
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</div>
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<div class="ddcolortabsline">&nbsp;</div>
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-
<div id="dropmenu1_a" class="dropmenudiv_a">
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<a href="https://2008.igem.org/Team:Edinburgh/Team">Overview</a>
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<a href="https://2008.igem.org/Team:Edinburgh/Team">Step1</a>
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<a href="https://2008.igem.org/Team:Edinburgh/Team">Step2</a>
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</div>
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<script type="text/javascript">
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-
//SYNTAX: tabdropdown.init("menu_id", [integer OR "auto"])
+
-
tabdropdown.init("colortab", 3)
+
-
</script>
+
-
</html>
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'''L1:''' Edinbrick1+''dxs'' (26.08.08)<br />
'''L1:''' Edinbrick1+''dxs'' (26.08.08)<br />
Line 304: Line 20:
'''L15:''' ligation of Edinbrick1 with rbs+''dxs'' fusion PCR product (P12). (14.07.08) - Failed<br />
'''L15:''' ligation of Edinbrick1 with rbs+''dxs'' fusion PCR product (P12). (14.07.08) - Failed<br />
'''L16:''' Ligation of Edinbrick1 with rbs+''appY'' fusion PCR product (P15) (17.07.08) - Failed<br />
'''L16:''' Ligation of Edinbrick1 with rbs+''appY'' fusion PCR product (P15) (17.07.08) - Failed<br />
-
'''L17:''' Ligation of ''crtB'' (M36) to rbs (22/07/08)<br />
+
'''L17:''' Ligation of rbs to pSB1A2+''crtB'' (M36) (22.07.08: OG, Yan)<br />
-
'''L18:''' Ligation of ''crtI'' (M42) to rbs (22/07/08)<br />
+
'''L18:''' Ligation of rbs to pSB1A2+''crtI'' (M42) (22.07.08: OG, Yan)<br />
-
'''L19:''' pSB1A2+rbs+''dxs'' ligation product (From P12) (25/07/08: Yan/OG)<br />
+
'''L19:''' pSB1A2+rbs+''dxs'' ligation product (From P12) (25.07.08: Yan, OG)<br />
-
'''L20:''' pSB1A2+rbs+''appY'' ligation product (From P15) (25/07/08: Yan/OG)<br />
+
'''L20:''' pSB1A2+rbs+''appY'' ligation product (From P15) (25.07.08: Yan, OG)<br />
-
'''L21:''' pSB1A2+rbs+''crtB'' ligation product (From P28) (25/07/08: Yan/OG)<br />
+
'''L21:''' pSB1A2+rbs+''crtB'' ligation product (From P28) (25.07.08: Yan, OG)<br />
-
'''L22:''' pSB1A2+rbs+''crtI'' ligation product (From P29) (25/07/08: Yan/OG)<br />
+
'''L22:''' pSB1A2+rbs+''crtI'' ligation product (From P29) (25.07.08: Yan, OG)<br />
-
'''L23:''' PzntA+pSB1A2 ligation product (From P31) (25/07/08: Yan/OG)<br />
+
'''L23:''' pSB1A2+pZntA ligation product (From P31) (25.07.08: Yan, OG)<br />
-
'''L24:''' Ligation of ''crtE'' to pSB1A2 (25/07/08: CF)<br />
+
'''L24:''' Ligation of ''crtE'' to pSB1A2 (25.07.08: CF)<br />
-
'''L25:''' Ligation of ''glgC'' double mutant (P36) to Edinbrick 1 (28.07.2008: AM)<br />
+
'''L25:''' Ligation of ''glgC''-mut1,2 (P36) to Edinbrick1, (28.07.2008: AM)<br />
-
'''L26:''' Ligation of Edi+P15 XP - rbs+''appY'' (30.07.2008: HX)<br />
+
'''L26:''' Ligation of P15 (rbs+''appY'') to Edinbrick1. (30.07.2008: HX)<br />
-
'''L27:''' Ligation of Edi+P28 XP - rbs+''crtB'' (30.07.2008: HX)<br />
+
'''L27:''' Ligation of P28 (rbs+''crtB'') to Edinbrick1. (30.07.2008: HX)<br />
-
'''L28:''' Putative ''cenA'' ligated to Edinbrick1 (30.07.08: AM)<br />
+
'''L28:''' Putative ''cenA'' ligated to Edinbrick1. (30.07.08: AM)<br />
-
'''L29:''' Putative ''cex'' ligated to Edinbrick1 (30.07.08: AM)<br />
+
'''L29:''' Putative ''cex'' ligated to Edinbrick1. (30.07.08: AM)<br />
-
'''L30:''' Self-ligation of P15 (3rd mutagenesis of ''glgC''-mut1,2) (05.08.08: HX)<br />
+
'''L30:''' Self-ligation of P50 (3rd mutagenesis of ''glgC''-mut1,2) (05.08.08: HX)<br />
'''L31:''' Ligation of P36 (''glgC''-mut1,2, hopefully, could be P39) to Edinbrick1 (05.08.08: OG)<br />
'''L31:''' Ligation of P36 (''glgC''-mut1,2, hopefully, could be P39) to Edinbrick1 (05.08.08: OG)<br />
-
'''L32:''' Ligation of rbs+''crtB'' (M67) to rbs+''crtI'' (M50) to create ''crtB''/''crtI'' (07.08.08: AM, Yan)<br />
+
'''L32:''' Ligation of rbs+''crtB'' (M67) to rbs+''crtI''+pSB1A2 (M50) to create pSB1A2+''crtB''+''crtI'' (07.08.08: AM, Yan)<br />
-
'''L33:''' Ligation of PcstA (P54) to Edinbrick1 (08.08.08: OG)<br />
+
'''L33:''' Ligation of PcstA (P54) to Edinbrick1. (08.08.08: OG)<br />
-
'''L34''': Combined dxs biobrick with CrtE biobrick (08.08.08: YAN)<br />
+
'''L34:''' Ligation of pSB1A2+rbs+''dxs'' with rbs+''crtE'' to make pSB1A2+''dxs''+''crtE''. (08.08.08: Yan)<br />
-
'''L35''': Combined dxs biobrick with Lims biobrick (08.08.08: YAN)<br />
+
'''L35:''' Ligation of pSB1A2+rbs+''dxs'' with rbs+''LIMS1'' to make pSB1A2+''dxs''+''crtE''.(08.08.08: Yan)<br />
-
'''L36''': crtY+pSB1A2 (P59 self-ligation) (09.08.08: CF)<br />
+
'''L36:''' pSB1A2+''crtY'' (P59 self-ligation). (09.08.08: CF)<br />
-
'''L37''': rbs+crtY+pSB1A2 (P60 self-ligation) (09.08.08: CF)<br />
+
'''L37:''' pSB1A2+rbs+''crtY'' (P60 self-ligation). (09.08.08: CF)<br />
-
'''L38''': crtE+pSB1A2 (M79 into Edinbrick1) (12.08.08: AH)<br />
+
'''L38''': ''crtE''+pSB1A2 (M79 into Edinbrick1) (12.08.08: AH)<br />
-
'''L39''': CeX into Edinbrick1 (13.08.08:YAN)<br />
+
'''L39''': ''cex'' into Edinbrick1 (13.08.08:YAN)<br />
-
'''L40''': CenA into Edinbrick1 (13.08.08:YAN)<br />
+
'''L40''': ''cenA'' into Edinbrick1 (13.08.08:YAN)<br />
-
'''L42''': dxs+lims (M137) to appY (M82). (18.08.08: YAN)<br />
+
'''L42''': ''dxs+LIMS'' (M137) to ''appY'' (M82). (18.08.08: YAN)<br />
-
'''L43''': crtB+crtI (M109) to appY (M82). (18.08.08: YAN)<br />
+
'''L43''': ''crtB+crtI'' (M109) to ''appY'' (M82). (18.08.08: YAN)<br />
-
'''L44''': pstA (M130) to lacB. (18.08.08: YAN)<br />
+
'''L44''': P''cstA'' (M130) to ''lacZ''. (18.08.08: YAN)<br />
-
'''L45''': glgC mut 1+2 (M71A) to SOB2 (18.08.08: AM)<br />
+
'''L45''': ''glgC'' mut 1+2 (M71A) to SOB2 (18.08.08: AM)<br />
-
'''L46''': glgC mut 1+2+3 (M71) to SOB2 (18.08.08: AM)<br />
+
'''L46''': ''glgC'' mut 1+2+3 (M71) to SOB2 (18.08.08: AM)<br />
-
'''L47''': glgC mut 1+2+3 (M72) to SOB2 (18.08.08: AM)<br />
+
'''L47''': ''glgC'' mut 1+2+3, a.k.a. ''glgC16'' (M72) to SOB2 (18.08.08: AM)<br />
-
'''L48''': Appy into dxs+lims (19.08.08 YAN)<br />
+
'''L48''': ''appY'' into ''dxs+LIMS'' (19.08.08 YAN)<br />
-
'''L49''': Appy into CrtBI (19.08.08 YAN)<br />
+
'''L49''': ''appY'' into ''crtBI'' (19.08.08 YAN)<br />
-
'''L50''': Self-ligation of P78 (SOB2-glgC mut 1+2) (20.08.08 AM)<br />
+
'''L50''': Self-ligation of P78 (SOB2-''glgC'' mut 1+2) (20.08.08 AM)<br />
-
'''L51''': Self-ligation of P79 (SOB2-glgC mut 1+2+3) (20.08.08 AM)<br />
+
'''L51''': Self-ligation of P79 (SOB2-''glgC'' mut 1+2+3) (20.08.08 AM)<br />
-
'''L52''': Self-ligation of P80 (SOB2-glgC mut 1+2+3) (20.08.08 AM)<br />
+
'''L52''': Self-ligation of P80 (SOB2-''glgC'' mut 1+2+3) (20.08.08 AM)<br />
-
'''L53''': Self-ligation of P81 (SOB2-rbs-glgC mut 1+2) (20.08.08 AM)<br />
+
'''L53''': Self-ligation of P81 (SOB2-rbs-''glgC'' mut 1+2) (20.08.08 AM)<br />
-
'''L54''': Self-ligation of P82 (SOB2-rbs-glgC mut 1+2+3) (20.08.08 AM)<br />
+
'''L54''': Self-ligation of P82 (SOB2-rbs-''glgC'' mut 1+2+3) (20.08.08 AM)<br />
-
'''L55''': Self-ligation of P83 (SOB2-rbs-glgC mut 1+2+3) (20.08.08 AM)<br />
+
'''L55''': Self-ligation of P83 (SOB2-rbs-''glgC'' mut 1+2+3) (20.08.08 AM)<br />
-
'''L56''': LacZ into PcstA (20.08.08 YAN)<br />
+
'''L56''': ''lacZ'' into P''cstA'' (20.08.08 YAN)<br />
 +
'''L57''': ''appY'' into ''crtBI'' (28.08.08 YAN)<br/>
 +
'''L58''': Self-ligation of P91 (03.09.08: YAN)<br/>
 +
'''L59''': Self-ligation of P92 (03.09.08: YAN)<br/>
 +
'''L60''': Self-ligation of P93 (03.09.08: YAN)<br/>
 +
'''L61''': Self-ligation of P94 (03.09.08: YAN)<br/>
 +
'''L62''': ''dxs+crtE'' into ''crtBI'' (09.09.08: YAN)<br/>
 +
'''L63''': P90 (''bglX'') + Edinbrick 1 (09.09.08: AM)<br/>
 +
'''L64''': P64 (''cenA'') + Edinbrick 1 (09.09.08: AM)<br/>
 +
'''L65''': P''lac''+ ''dxs+LIMS+appY'' (CF)<br/>
 +
'''L66''': P''cstA''+''lacZ'' (CF)<br/>
 +
'''L68''': self-ligation of P91 (sob-''SU1'')(CF, 22-9-8)<br/>
 +
'''L69''': self-ligation of P93 (sob-''ISO2'')(CF, 23-9-8)<br/>
 +
'''L70''': self-ligation of P96 (mutagenesis of ''cex'')(CF, 23-9-8)<br/>
 +
'''L73''': ligation of P65 (''cenA'') to Edinbrick1. (CF, 24-9-8)<br/>
 +
'''L74''': ligation of P90 (''bglX'') to Edinbrick1. (CF, 24-9-8)<br/>
 +
'''L75''': ligation of P99 (rbs+''glgC'') to Edinbrick1. (CF, 25-9-8)<br/>
 +
'''L76''': ligation of P100 (rbs+''glgC16'') to Edinbrick1. (CF, 25-9-8)<br/>
 +
'''L77''': ligation of ''crtBI'' to ''dxs.crtE''. (CF, 26-9-8)<br/>
 +
'''L78''': ligation of ''xylE'' (BBa_J33204) to ''PcstA''. (CF, 29-9-8)<br/>
 +
'''L79''': ligation of P''lac+lacZ' '' (BBa_J33207) to rbs+''glgC''. (CF, 29-9-8)<br/>
 +
'''L80''': ligation of P''lac+lacZ' '' (BBa_J33207) to rbs+''glgC16''. (CF, 29-9-8)<br/>
 +
'''L81''': ligation of rbs to ''cex''. (CF, 30-9-8)<br/>
 +
'''L82''': ligation of rbs to ''cenA''. (CF, 30-9-8)<br/>
 +
'''L83''': ligation of P''lac+lacZ' '' (BBa_J33207) to ''crtBI''. (CF, 30-9-8)<br/>
 +
'''L84''': ligation of P''lac+lacZ' '' (BBa_J33207) to ''dxs.crtE''. (CF 30-9-8)<br/>
 +
'''L87''': ligation of ''bglX'' to Edinbrick2. (CF, 7-10-8)<br/>
 +
'''L88''': ligation of P105 (''bglX'' with complete suffix) to Edinbrick1. (CF, 10-10-8)<br/>
 +
'''L89''': ligation of P105 (''bglX'' with complete suffix) to Edinbrick2. (CF, 10-10-8)<br/>
 +
'''L90''': self-ligation of P107, mutagenesis of ''SU1''. (CF, 10-10-8)<br/>
 +
'''L91''': self-ligation of P107, mutagenesis of ''ISO2''. (CF, 11-10-8)<br/>
 +
'''L92''': repeat L83 (CF, 12-10-8)<br/>
 +
'''L93''': repeat L84 (CF, 12-10-8)<br/>
 +
'''L94''': ligation of P''lac+lacZ' '' (BBa_J33207) to rbs+''cenA'' (later turned out to be wrong clone). (CF, 29-9-8)<br/>
 +
'''L95''': ligation of P''lac+lacZ' '' (BBa_J33207) to rbs+''cex'' (later turned out to be wrong clone). (CF, 29-9-8)<br/>
 +
'''L96''': ligation of P''lac+crtBI'' to ''dxs.crtE''. (CF, 16-10-8)<br/>
 +
'''L97''': ligation of P''lac+dxs.crtE'' to ''crtBI''. (CF, 16-10-8)<br/>
 +
'''L98''': repeat of L90. (CF, 16-10-8)<br/>
 +
'''L99''': repeat of L91. (CF, 16-10-8)<br/>
 +
'''L100''': ligation of rbs to ''bglX''. (CF, 22-10-8)<br/>
 +
'''L101''': ligation of P110 (rbs+''cex'') to Edinbrick1. (CF, 23-10-8).<br/>
 +
'''L102''': ligation of P111 (rbs+''cenA'') to Edinbrick1. (CF, 23-10-8).<br/>
 +
'''L103''': ligation of P112 (rbs+''bglX'') to Edinbrick1. (CF, 23-10-8).<br/>
 +
'''L104''': ligation of P113 (''dxs.crtEBI'') to Edinbrick1. (CF, 27-10-8).<br/>

Latest revision as of 23:39, 29 October 2008

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Ligation Products

L1: Edinbrick1+dxs (26.08.08)
L2: Edinbrick1+appY (26.08.08)
L3: Babel1+glgC (26.08.08)
L4: Babel2+glgC (26.08.08)
L5: Edinbrick1+appY repeat (initially labelled L4 in error) (04.07.08)
L6: Self ligation of mutagenic PCR P6 (BABEL2+glgC-mut1). (07.07.08)
L7: Self ligation of mutagenic PCR P7 (BABEL2+glgC-mut2). (07.07.08)
L8: Ligation of synthetic ribosome binding site to M2 (pSB1A2+dxs, BBa_K118000). (09.07.08)
L9: Ligation of Edinbrick1 with crtB. (10.07.08)
L10: Ligation of Edinbrick1 with crtI (mutant lacking PstI sites, I hope). (10.07.08)
L11: repeat ligation of synthetic ribosome binding site to M2 (pSB1A2+dxs, BBa_K118000). (11.07.08: Yan, HX)
L12: rbs ligated with pSB1A2+appY (M15). (14.07.08)
L13 and L14: self ligations of P13 and P14 (BABEL2+glgC-mut1,2). (14.07.08)
L15: ligation of Edinbrick1 with rbs+dxs fusion PCR product (P12). (14.07.08) - Failed
L16: Ligation of Edinbrick1 with rbs+appY fusion PCR product (P15) (17.07.08) - Failed
L17: Ligation of rbs to pSB1A2+crtB (M36) (22.07.08: OG, Yan)
L18: Ligation of rbs to pSB1A2+crtI (M42) (22.07.08: OG, Yan)
L19: pSB1A2+rbs+dxs ligation product (From P12) (25.07.08: Yan, OG)
L20: pSB1A2+rbs+appY ligation product (From P15) (25.07.08: Yan, OG)
L21: pSB1A2+rbs+crtB ligation product (From P28) (25.07.08: Yan, OG)
L22: pSB1A2+rbs+crtI ligation product (From P29) (25.07.08: Yan, OG)
L23: pSB1A2+pZntA ligation product (From P31) (25.07.08: Yan, OG)
L24: Ligation of crtE to pSB1A2 (25.07.08: CF)
L25: Ligation of glgC-mut1,2 (P36) to Edinbrick1, (28.07.2008: AM)
L26: Ligation of P15 (rbs+appY) to Edinbrick1. (30.07.2008: HX)
L27: Ligation of P28 (rbs+crtB) to Edinbrick1. (30.07.2008: HX)
L28: Putative cenA ligated to Edinbrick1. (30.07.08: AM)
L29: Putative cex ligated to Edinbrick1. (30.07.08: AM)
L30: Self-ligation of P50 (3rd mutagenesis of glgC-mut1,2) (05.08.08: HX)
L31: Ligation of P36 (glgC-mut1,2, hopefully, could be P39) to Edinbrick1 (05.08.08: OG)
L32: Ligation of rbs+crtB (M67) to rbs+crtI+pSB1A2 (M50) to create pSB1A2+crtB+crtI (07.08.08: AM, Yan)
L33: Ligation of PcstA (P54) to Edinbrick1. (08.08.08: OG)
L34: Ligation of pSB1A2+rbs+dxs with rbs+crtE to make pSB1A2+dxs+crtE. (08.08.08: Yan)
L35: Ligation of pSB1A2+rbs+dxs with rbs+LIMS1 to make pSB1A2+dxs+crtE.(08.08.08: Yan)
L36: pSB1A2+crtY (P59 self-ligation). (09.08.08: CF)
L37: pSB1A2+rbs+crtY (P60 self-ligation). (09.08.08: CF)
L38: crtE+pSB1A2 (M79 into Edinbrick1) (12.08.08: AH)
L39: cex into Edinbrick1 (13.08.08:YAN)
L40: cenA into Edinbrick1 (13.08.08:YAN)
L42: dxs+LIMS (M137) to appY (M82). (18.08.08: YAN)
L43: crtB+crtI (M109) to appY (M82). (18.08.08: YAN)
L44: PcstA (M130) to lacZ. (18.08.08: YAN)
L45: glgC mut 1+2 (M71A) to SOB2 (18.08.08: AM)
L46: glgC mut 1+2+3 (M71) to SOB2 (18.08.08: AM)
L47: glgC mut 1+2+3, a.k.a. glgC16 (M72) to SOB2 (18.08.08: AM)
L48: appY into dxs+LIMS (19.08.08 YAN)
L49: appY into crtBI (19.08.08 YAN)
L50: Self-ligation of P78 (SOB2-glgC mut 1+2) (20.08.08 AM)
L51: Self-ligation of P79 (SOB2-glgC mut 1+2+3) (20.08.08 AM)
L52: Self-ligation of P80 (SOB2-glgC mut 1+2+3) (20.08.08 AM)
L53: Self-ligation of P81 (SOB2-rbs-glgC mut 1+2) (20.08.08 AM)
L54: Self-ligation of P82 (SOB2-rbs-glgC mut 1+2+3) (20.08.08 AM)
L55: Self-ligation of P83 (SOB2-rbs-glgC mut 1+2+3) (20.08.08 AM)
L56: lacZ into PcstA (20.08.08 YAN)
L57: appY into crtBI (28.08.08 YAN)
L58: Self-ligation of P91 (03.09.08: YAN)
L59: Self-ligation of P92 (03.09.08: YAN)
L60: Self-ligation of P93 (03.09.08: YAN)
L61: Self-ligation of P94 (03.09.08: YAN)
L62: dxs+crtE into crtBI (09.09.08: YAN)
L63: P90 (bglX) + Edinbrick 1 (09.09.08: AM)
L64: P64 (cenA) + Edinbrick 1 (09.09.08: AM)
L65: Plac+ dxs+LIMS+appY (CF)
L66: PcstA+lacZ (CF)
L68: self-ligation of P91 (sob-SU1)(CF, 22-9-8)
L69: self-ligation of P93 (sob-ISO2)(CF, 23-9-8)
L70: self-ligation of P96 (mutagenesis of cex)(CF, 23-9-8)
L73: ligation of P65 (cenA) to Edinbrick1. (CF, 24-9-8)
L74: ligation of P90 (bglX) to Edinbrick1. (CF, 24-9-8)
L75: ligation of P99 (rbs+glgC) to Edinbrick1. (CF, 25-9-8)
L76: ligation of P100 (rbs+glgC16) to Edinbrick1. (CF, 25-9-8)
L77: ligation of crtBI to dxs.crtE. (CF, 26-9-8)
L78: ligation of xylE (BBa_J33204) to PcstA. (CF, 29-9-8)
L79: ligation of Plac+lacZ' (BBa_J33207) to rbs+glgC. (CF, 29-9-8)
L80: ligation of Plac+lacZ' (BBa_J33207) to rbs+glgC16. (CF, 29-9-8)
L81: ligation of rbs to cex. (CF, 30-9-8)
L82: ligation of rbs to cenA. (CF, 30-9-8)
L83: ligation of Plac+lacZ' (BBa_J33207) to crtBI. (CF, 30-9-8)
L84: ligation of Plac+lacZ' (BBa_J33207) to dxs.crtE. (CF 30-9-8)
L87: ligation of bglX to Edinbrick2. (CF, 7-10-8)
L88: ligation of P105 (bglX with complete suffix) to Edinbrick1. (CF, 10-10-8)
L89: ligation of P105 (bglX with complete suffix) to Edinbrick2. (CF, 10-10-8)
L90: self-ligation of P107, mutagenesis of SU1. (CF, 10-10-8)
L91: self-ligation of P107, mutagenesis of ISO2. (CF, 11-10-8)
L92: repeat L83 (CF, 12-10-8)
L93: repeat L84 (CF, 12-10-8)
L94: ligation of Plac+lacZ' (BBa_J33207) to rbs+cenA (later turned out to be wrong clone). (CF, 29-9-8)
L95: ligation of Plac+lacZ' (BBa_J33207) to rbs+cex (later turned out to be wrong clone). (CF, 29-9-8)
L96: ligation of Plac+crtBI to dxs.crtE. (CF, 16-10-8)
L97: ligation of Plac+dxs.crtE to crtBI. (CF, 16-10-8)
L98: repeat of L90. (CF, 16-10-8)
L99: repeat of L91. (CF, 16-10-8)
L100: ligation of rbs to bglX. (CF, 22-10-8)
L101: ligation of P110 (rbs+cex) to Edinbrick1. (CF, 23-10-8).
L102: ligation of P111 (rbs+cenA) to Edinbrick1. (CF, 23-10-8).
L103: ligation of P112 (rbs+bglX) to Edinbrick1. (CF, 23-10-8).
L104: ligation of P113 (dxs.crtEBI) to Edinbrick1. (CF, 27-10-8).

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