IGEM:Cambridge/2008/Notebook/Voltage/Future Work
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(New page: =Move to ''Bacillus''= Need to make GluR0 bacillus compatible *Codon bias already favourable for ''Bacillus'') |
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- | =Move to [[Team:Cambridge/Bacillus|''Bacillus'']]= | + | =Future Work= |
- | Need to make GluR0 | + | |
+ | ==Better K+ uptake== | ||
+ | If a stronger K+ uptake could be made then in pricipal cells could be grown in the same medium as testing was done | ||
+ | *Up regulate KDP constitutively | ||
+ | *Find K+ tolerant strains | ||
+ | *Check maximum trans-membrane potential | ||
+ | |||
+ | ==Move to [[Team:Cambridge/Bacillus|''Bacillus'']]== | ||
+ | Need to make GluR0 ''Bacillus'' compatible | ||
*Codon bias already favourable for ''Bacillus'' | *Codon bias already favourable for ''Bacillus'' | ||
+ | *Need to put different promoter and RBS | ||
+ | *Integrate into genome? | ||
+ | |||
+ | ==Voltage Gated Potassium Channel== | ||
+ | To get self propogating signal we need to find and construct a voltage gated potassium channel. | ||
+ | *Relatively rare in Prokaryotes | ||
+ | *Some available although not well characterised, such as Kch | ||
+ | *Overexpress and see if it improves output. |
Revision as of 20:28, 29 October 2008
Contents |
Future Work
Better K+ uptake
If a stronger K+ uptake could be made then in pricipal cells could be grown in the same medium as testing was done
- Up regulate KDP constitutively
- Find K+ tolerant strains
- Check maximum trans-membrane potential
Move to Bacillus
Need to make GluR0 Bacillus compatible
- Codon bias already favourable for Bacillus
- Need to put different promoter and RBS
- Integrate into genome?
Voltage Gated Potassium Channel
To get self propogating signal we need to find and construct a voltage gated potassium channel.
- Relatively rare in Prokaryotes
- Some available although not well characterised, such as Kch
- Overexpress and see if it improves output.