Illinois/1 June 2008

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(New page: *Receptor Tyrosine Kinase method: Made Yeast Media YPD Medium, per liter: 10g yeast extract 20g peptone 20g dextrose 20g agar(only for plates) 1. Dextrose filter sterilized, the rest aut...)
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*Receptor Tyrosine Kinase method:
 
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Made Yeast Media
 
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YPD Medium, per liter:
 
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10g yeast extract
 
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20g peptone
 
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20g dextrose
 
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20g agar(only for plates)
 
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1. Dextrose filter sterilized, the rest autoclaved
 
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2. Weigh nutrients into flask double the volume you want to make, and stir to dissolve
 
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3. Dextrose added to autoclaved media to equivalent of 20 g/L
 
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4. Liquid media placed on bench, plate media placed in 65 degree water bath approximately 5 minutes
 
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5. Poured into plates and allowed to solidify
 

Latest revision as of 15:39, 30 July 2008