http://2008.igem.org/wiki/index.php?title=Jamboree/Project_Abstract/Team_Abstracts&feed=atom&action=historyJamboree/Project Abstract/Team Abstracts - Revision history2024-03-29T00:32:13ZRevision history for this page on the wikiMediaWiki 1.16.5http://2008.igem.org/wiki/index.php?title=Jamboree/Project_Abstract/Team_Abstracts&diff=105903&oldid=prevMeagan at 15:27, 6 November 20082008-11-06T15:27:17Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Microorganisms typically preferentially utilize glucose over other sugar carbon sources such as xylose. This is largely regulated through control of gene expression based on the response of regulatory elements to sugars available to the cell. In E. coli, the xylose metabolism operon is controlled by both the xylose-inducible XylR activator protein and the cAMP receptor protein (CRP). In this project we attempt to eliminate glucose control over xylose-inducible gene expression in E. coli by altering the natural transcriptional control region of the xylose operon. Designs constructed and tested include scrambling the CRP binding site, increasing the strength of the xyl promoter, and over expressing XylR. Xylose-inducible gene expression that functions independently of glucose regulation provides a useful approach to improving microbial utilization of biomass feedstocks containing mixtures of glucose and xylose.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Microorganisms typically preferentially utilize glucose over other sugar carbon sources such as xylose. This is largely regulated through control of gene expression based on the response of regulatory elements to sugars available to the cell. In E. coli, the xylose metabolism operon is controlled by both the xylose-inducible XylR activator protein and the cAMP receptor protein (CRP). In this project we attempt to eliminate glucose control over xylose-inducible gene expression in E. coli by altering the natural transcriptional control region of the xylose operon. Designs constructed and tested include scrambling the CRP binding site, increasing the strength of the xyl promoter, and over expressing XylR. Xylose-inducible gene expression that functions independently of glucose regulation provides a useful approach to improving microbial utilization of biomass feedstocks containing mixtures of glucose and xylose.</div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">====[[Team:Prairie_View | Prairie View]]====</ins></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">''Modeling Molecular Biosensor: Use of eNOSE and Neural Network System''</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">Biosensors are functional molecules and/or cells including microbial cells that allow detection of the presence of different molecules and/or metal ions such as iron , vanadium, nickel, and other elements, even at detection levels beyond limits of conventional methods. Therefore, the aim of the denoted project was to disign a divice for detection of different levels of Fe (II), Ni (II), and V (II). The response of the biosensor was measured by DNA and protein fluorescence, bacterial growth (CFU), and ATP production. The device was tested at different concentrations of the metal ions.A computational modeling, neural network system coupled to an eNose system was developed to accurately assemble and predict the efficacy of the final biosensor device.</ins></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">====[[Team:Princeton | Princeton]]====</ins></div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">''Genetically engineered neuronal circuits: the fast and the furious''</ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">The electrical and chemical excitability of biological neurons make them excellent components for synthetic biology systems. We designed and partially constructed genetic programs that drive the formation of several specific neuronal cell types from embryonic stem cells. A two-phase genetic program is used to first drive stem cell differentiation into neuronal precursors followed by differentiation into mature neurons that synthesize and respond to specific neurotransmitters. We arrange populations of three types of genetically engineered neurons in a topology that implements a (very fast) bi-stable toggle switch. Pacemaker cells serve as the ‘power source’ and constantly transmit excitatory dopamine-based action potentials to the other two cells types. These two cell types cross-repress each other using inhibitory neurotransmitters (e.g. GABA and glycine) such that only of these cell types is active. The system is switched between the two stable states through external induction with the inhibitory neurotransmitters.</ins></div></td></tr>
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</table>Meaganhttp://2008.igem.org/wiki/index.php?title=Jamboree/Project_Abstract/Team_Abstracts&diff=105896&oldid=prevMeagan at 21:48, 5 November 20082008-11-05T21:48:10Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>__NOTOC__</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>__NOTOC__</div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;"><span style="color:#555555; font-family:georgia, serif; font-weight:bold;">Download the iGEM 2008 Jamboree Program [https://static.igem.org/mediawiki/2008/6/6c/Igem_08_program.pdf here].</span></ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====[[Team:Alberta NINT | Alberta NINT]]====</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====[[Team:Alberta NINT | Alberta NINT]]====</div></td></tr>
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</table>Meaganhttp://2008.igem.org/wiki/index.php?title=Jamboree/Project_Abstract/Team_Abstracts&diff=62612&oldid=prevMsicchio: /* Warsaw */2008-10-24T16:51:26Z<p><span class="autocomment">Warsaw</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Bacterial device for creating and production of interactors for any given bait protein''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Bacterial device for creating and production of interactors for any given bait protein''</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>We have developed a system allowing to search for antibodies with new specificities or screen protein libraries in order to generate interactors for a given bait. Our system changes protein sequence to maximize its interaction with a given partner. Proteins with modified <del class="diffchange diffchange-inline">sequence </del>are then directed to bacterial outer membrane, where the best interactors are selected. Protein presented on the cell surface is fused with part of β-lactamase protein, while its bait is fused with complementing part of the enzyme and added to medium. The stronger interaction between proteins of interest, the more efficient binding of the two halves of β-lactamase, leading to resistance to ampicillin and survival. Cells expressing less interacting variants die as they don't achieve sufficient complementation of the reporter enzyme. This allows us to select strains producing interactors for any given bait.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>We have developed a system allowing to search for antibodies with new specificities or screen protein libraries in order to generate interactors for a given bait. Our system changes <ins class="diffchange diffchange-inline">a </ins>protein sequence to maximize its interaction with a given partner. Proteins with modified <ins class="diffchange diffchange-inline">sequences </ins>are then directed to <ins class="diffchange diffchange-inline">the </ins>bacterial outer membrane, where the best interactors are selected. Protein presented on the cell surface is fused with part of β-lactamase protein, while its bait is fused with <ins class="diffchange diffchange-inline">the </ins>complementing part of the enzyme and added to medium. The stronger <ins class="diffchange diffchange-inline">the </ins>interaction between proteins of interest, the more efficient <ins class="diffchange diffchange-inline">the </ins>binding of the two halves of β-lactamase, leading to resistance to ampicillin and survival. Cells expressing less interacting variants die as they don't achieve sufficient complementation of the reporter enzyme. This allows us to select strains producing interactors for any given bait.</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====[[Team:Waterloo | Waterloo]]====</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====[[Team:Waterloo | Waterloo]]====</div></td></tr>
</table>Msicchiohttp://2008.igem.org/wiki/index.php?title=Jamboree/Project_Abstract/Team_Abstracts&diff=62602&oldid=prevMsicchio: /* Valencia */2008-10-24T16:48:57Z<p><span class="autocomment">Valencia</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''The Hot Yeast Project: Heat production in UCP-1-expressing yeasts''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''The Hot Yeast Project: Heat production in UCP-1-expressing yeasts''</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The present project aims to demonstrate that the temperature of a microbial culture might be modulated through the expression of the mammalian uncoupling protein UCP-1. Saccharomyces cerevisiae strains genetically modified to express wild type UCP-1, mutant sequences with increased uncoupling activity, as well as a control strain were cultured in an Liquid Culture Calorimeter (LCC) we developed. The system consisted of a modified thermo flask with an inserted thermocouple allowing real-time accurate temperature measurements. Different conditions, such as initial densities, amounts of inductor or shaking speeds were tested. We succeeded to obtain significant temperature increases in the mutant strains compared with the other strains. We also developed an effective model of our system. Although the system is not always stable and might be sensitive to external perturbations, this is the first time a significant increase in temperature associated to UCP-1 expression in yeast is reported.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The present project aims to demonstrate that the temperature of a microbial culture might be modulated through the expression of the mammalian uncoupling protein UCP-1. Saccharomyces cerevisiae strains genetically modified to express wild type UCP-1, mutant sequences with increased uncoupling activity, as well as a control strain were cultured in an Liquid Culture Calorimeter (LCC) we developed. The system consisted of a modified thermo flask with an inserted thermocouple allowing real-time accurate temperature measurements. Different conditions, such as initial densities, amounts of inductor<ins class="diffchange diffchange-inline">, </ins>or shaking speeds were tested. We succeeded to obtain significant temperature increases in the mutant strains compared with the other strains. We also developed an effective model of our system. Although the system is not always stable and might be sensitive to external perturbations, this is the first time a significant increase in temperature associated to UCP-1 expression in yeast is reported.</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====[[Team:Virginia | Virginia]]====</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====[[Team:Virginia | Virginia]]====</div></td></tr>
</table>Msicchiohttp://2008.igem.org/wiki/index.php?title=Jamboree/Project_Abstract/Team_Abstracts&diff=62574&oldid=prevMsicchio: /* University of Sheffield */2008-10-24T16:44:21Z<p><span class="autocomment">University of Sheffield</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Fusion receptors – an approach to confer new features on bacteria''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Fusion receptors – an approach to confer new features on bacteria''</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Expression of non-native receptor proteins in bacteria often involves extensive genetic modifications that can be difficult to execute. One way of addressing this problem is making fusion receptor protein consisting of the sensing part derived from a foreign species, and a signal transmitting part that is native to the organism in which receptor is to be expressed. The fusion receptor we have designed consists of Vibrio cholerae's sensing module fused to E.coli's signal conveyer. <del class="diffchange diffchange-inline">Receiver </del>part of a receptor should bind to signaling molecules excreted by V.cholera and pass it downstream through signal <del class="diffchange diffchange-inline">transmitter </del>to DNA. Expression of reporter <del class="diffchange diffchange-inline">molecule </del>will indicate water contamination. As a result the fusion receptor could be applied in real life as a basis for cheap device sensing water contamination.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Expression of non-native receptor proteins in bacteria often involves extensive genetic modifications that can be difficult to execute. One way of addressing this problem is making <ins class="diffchange diffchange-inline">a </ins>fusion receptor protein consisting of the sensing part derived from a foreign species, and a signal transmitting part that is native to the organism in which <ins class="diffchange diffchange-inline">the </ins>receptor is to be expressed. The fusion receptor we have designed consists of Vibrio cholerae's sensing module fused to E.coli's signal conveyer. <ins class="diffchange diffchange-inline">The receiver </ins>part of a receptor should bind to signaling molecules excreted by V.cholera and pass it downstream through signal <ins class="diffchange diffchange-inline">transmitters </ins>to DNA. Expression of reporter <ins class="diffchange diffchange-inline">molecules </ins>will indicate water contamination. As a result<ins class="diffchange diffchange-inline">, </ins>the fusion receptor could be applied in real life as a basis for <ins class="diffchange diffchange-inline">a </ins>cheap device sensing water contamination.</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====[[Team:University of Washington | University of Washington]]====</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====[[Team:University of Washington | University of Washington]]====</div></td></tr>
</table>Msicchiohttp://2008.igem.org/wiki/index.php?title=Jamboree/Project_Abstract/Team_Abstracts&diff=62551&oldid=prevMsicchio: /* TUDelft */2008-10-24T16:39:17Z<p><span class="autocomment">TUDelft</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Engineering Bio-thermometers at Delft University of Technology''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Engineering Bio-thermometers at Delft University of Technology''</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The goal of our project is to construct temperature-sensing bacteria Escherichia coli that changes color at different temperatures. Such a thermometer can be applied <del class="diffchange diffchange-inline">e.g. </del>as a temperature reporter system in large-scale fermentations, or as a temperature-inducible protein production system. The functionality of this thermometer relies on the post-transcriptional regulation of a temperature-sensitive RNA structure<del class="diffchange diffchange-inline">: it </del>opens and enables the ribosome to bind, only when the temperature exceeds a certain threshold. We designed new artificial temperature sensitive RNA sequences, and developed protocols, using luciferase as a reporter, to test their functionality. For the colour output, we built upon the existing carotene biosynthesis pathway and converted all new elements to the BioBrick standard. Furthermore, we developed mathematical models describing both the temperature sensitive parts and the colour mevalonate pathway, and estimated parameters using the experimental data. The ethical issues in design and possible implementation of a commercial product are also addressed. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The goal of our project is to construct temperature-sensing bacteria Escherichia coli that changes color at different temperatures. Such a thermometer can be applied as a temperature reporter system in large-scale fermentations, or as a temperature-inducible protein production system. The functionality of this thermometer relies on the post-transcriptional regulation of a temperature-sensitive RNA structure<ins class="diffchange diffchange-inline">. It </ins>opens and enables the ribosome to bind, only when the temperature exceeds a certain threshold. We designed new artificial temperature sensitive RNA sequences, and developed protocols, using luciferase as a reporter, to test their functionality. For the colour output, we built upon the existing carotene biosynthesis pathway and converted all new elements to the BioBrick standard. Furthermore, we developed mathematical models describing both the temperature sensitive parts and the colour mevalonate pathway, and estimated parameters using the experimental data. The ethical issues in design and possible implementation of a commercial product are also addressed.</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====[[Team:UC Berkeley | UC Berkeley]]====</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====[[Team:UC Berkeley | UC Berkeley]]====</div></td></tr>
</table>Msicchiohttp://2008.igem.org/wiki/index.php?title=Jamboree/Project_Abstract/Team_Abstracts&diff=62542&oldid=prevMsicchio: /* Tsinghua */2008-10-24T16:37:36Z<p><span class="autocomment">Tsinghua</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Modeling and reconstruction of the Escherichia coli chemotaxis system/Construction of a Polyhydroxyalkanoates(PHA) production induced-lysis cell''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Modeling and reconstruction of the Escherichia coli chemotaxis system/Construction of a Polyhydroxyalkanoates(PHA) production induced-lysis cell''</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>1:Inspired by the chemotaxis system of bacteria, we isolated and reconstructed a set of genetic modules in order to reconstitute an independent and interchangeable chemotactic device used as pollutant detector. Novel cybernetics terms and methods are introduced <del class="diffchange diffchange-inline">in </del>while in silico modeling together with related softwares are also established to simulate the effects.<br> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>1:Inspired by the chemotaxis system of bacteria, we isolated and reconstructed a set of genetic modules in order to reconstitute an independent and interchangeable chemotactic device used as pollutant detector. Novel cybernetics terms and methods are introduced<ins class="diffchange diffchange-inline">, </ins>while in silico modeling together with related softwares are also established to simulate the effects.<br> </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>2:In this project we are going to establish a novel bacteria strain which will sense the production of PHA, a degradable material used in environmentally friendly plastics. The key of this construction is to find a link between the amount of PHA particles and gene expression. A wildtype circuit and an artificial device are combined together to achieve this purpose. Lysis genes from phage are introduced to break the cell and release the particles. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>2:In this project we are going to establish a novel bacteria strain which will sense the production of PHA, a degradable material used in environmentally friendly plastics. The key of this construction is to find a link between the amount of PHA particles and gene expression. A wildtype circuit and an artificial device are combined together to achieve this purpose. Lysis genes from <ins class="diffchange diffchange-inline">the </ins>phage are introduced to break the cell and release the particles.</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====[[Team:TUDelft | TUDelft]]====</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====[[Team:TUDelft | TUDelft]]====</div></td></tr>
</table>Msicchiohttp://2008.igem.org/wiki/index.php?title=Jamboree/Project_Abstract/Team_Abstracts&diff=62529&oldid=prevMsicchio: /* Tokyo Tech */2008-10-24T16:34:17Z<p><span class="autocomment">Tokyo Tech</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Coli.Touch – implementation of a pressure-responsive genetic circuit in E. Coli''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''Coli.Touch – implementation of a pressure-responsive genetic circuit in E. Coli''</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Our project is to construct a bacterial `touch panel’ which is colored by the pressure. We name it E. coli touch or Coli. touch. In iGEM, genetic circuits <del class="diffchange diffchange-inline">responded by </del>various inputs -- <del class="diffchange diffchange-inline">for example, </del>heat, small molecule, and light -- have been constructed. However, a pressure-responsive genetic circuit <del class="diffchange diffchange-inline">had </del>not been constructed yet. Therefore, we constructed a pressure responsive circuit using a pressure-inducible promoter. Under high pressure, the affinity of LacI for the lac operator in lac promoter is known to <del class="diffchange diffchange-inline">decreases </del>due to a tetramer to dimer transition of LacI. However, we need 30 MPa pressure for induction of the lac promoter. Therefore, we created a withstand high-pressure display, and we tried to create a promoter induced by the lower pressure. In order to implement rewritable function in Coli. touch, we are planning to construct a toggle switch circuit using the lac promoter.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Our project is to construct a bacterial `touch panel’ which is colored by the pressure. We name it E. coli touch or Coli. touch. In iGEM, genetic circuits <ins class="diffchange diffchange-inline">that respond to </ins>various inputs -- heat, small molecule, and light -- have been constructed. However, a pressure-responsive genetic circuit <ins class="diffchange diffchange-inline">has </ins>not been constructed yet. Therefore, we constructed a pressure responsive circuit using a pressure-inducible promoter. Under high pressure, the affinity of LacI for the lac operator in lac promoter is known to <ins class="diffchange diffchange-inline">decrease </ins>due to a tetramer to dimer transition of LacI. However, we need 30 MPa pressure for induction of the lac promoter. Therefore, we created a withstand high-pressure display, and we tried to create a promoter induced by the lower pressure. In order to implement rewritable function in Coli. touch, we are planning to construct a toggle switch circuit using the lac promoter.</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====[[Team:Tsinghua | Tsinghua]]====</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====[[Team:Tsinghua | Tsinghua]]====</div></td></tr>
</table>Msicchiohttp://2008.igem.org/wiki/index.php?title=Jamboree/Project_Abstract/Team_Abstracts&diff=62523&oldid=prevMsicchio: /* Tianjin */2008-10-24T16:32:11Z<p><span class="autocomment">Tianjin</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''A synthetic convertible ecosystem & A foolproof genetic self-assembly system ''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''A synthetic convertible ecosystem & A foolproof genetic self-assembly system ''</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Tianjin’s program is composed of two projects: in project #1 a Prisoner’s Dilemma will be imposed to two cocultured strains of E coli, while in project #2, an effort has been made to improve the methodology of gene cloning experiments<del class="diffchange diffchange-inline">: </del>#1. A bistable ecosystem comprised of two strains that could switch between mutualism and competition has been built. The relationship between the two could be regulated by changing culture conditions. By doing this, we explored the possibilities of improving the coexistent ecosystems that function in industries<del class="diffchange diffchange-inline">, </del>#2. A genetic self-assembly system was built to reduce the labor and cost involved in gene cloning experiments. Via the mechanism of site-specific recombination and incompatibility of plasmids, our device could make it possible that the recombination of the genes of interest as well as the dilution of the undesired recombinant genes will be automatically performed by the cells, upon introducing the foreign genes. <del class="diffchange diffchange-inline"> </del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Tianjin’s program is composed of two projects: in project #1 a Prisoner’s Dilemma will be imposed to two cocultured strains of E coli, while in project #2, an effort has been made to improve the methodology of gene cloning experiments<ins class="diffchange diffchange-inline">. </ins>#1. A bistable ecosystem comprised of two strains that could switch between mutualism and competition has been built. The relationship between the two could be regulated by changing culture conditions. By doing this, we explored the possibilities of improving the coexistent ecosystems that function in industries<ins class="diffchange diffchange-inline">. </ins>#2. A genetic self-assembly system was built to reduce the labor and cost involved in gene cloning experiments. Via the mechanism of site-specific recombination and incompatibility of plasmids, our device could make it possible that the recombination of the genes of interest as well as the dilution of the undesired recombinant genes will be automatically performed by the cells, upon introducing the foreign genes.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====[[Team:Tokyo Tech | Tokyo Tech]]====</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====[[Team:Tokyo Tech | Tokyo Tech]]====</div></td></tr>
</table>Msicchiohttp://2008.igem.org/wiki/index.php?title=Jamboree/Project_Abstract/Team_Abstracts&diff=62519&oldid=prevMsicchio: /* Rice University */2008-10-24T16:30:59Z<p><span class="autocomment">Rice University</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''BioBeer''</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>''BioBeer''</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Resveratrol, a phytochemical used for defense in plants, has been implicated as a natural product that increases life span and prevents cancer. Unfortunately, significant levels of resveratrol are present in only a small number of foods, such as red wine, peanuts, and blueberries. To create an alternative source for resveratrol consumption, we are introducing a biosynthetic pathway for this compound into a brewing strain of <del class="diffchange diffchange-inline">/</del>Saccharomyces cerevisiae <del class="diffchange diffchange-inline">/</del>and examining whether this strain can be engineered to produce resveratrol during beer fermentation. Given the high worldwide consumption of beer and the low cost of production, unfiltered beer brewed using our genetically modified <del class="diffchange diffchange-inline">/</del>S. cerevisiae <del class="diffchange diffchange-inline">/</del>should provide a cost-effective source of pharmacologically-active resveratrol.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Resveratrol, a phytochemical used for defense in plants, has been implicated as a natural product that increases life span and prevents cancer. Unfortunately, significant levels of resveratrol are present in only a small number of foods, such as red wine, peanuts, and blueberries. To create an alternative source for resveratrol consumption, we are introducing a biosynthetic pathway for this compound into a brewing strain of <ins class="diffchange diffchange-inline">''</ins>Saccharomyces cerevisiae<ins class="diffchange diffchange-inline">'' </ins>and examining whether this strain can be engineered to produce resveratrol during beer fermentation. Given the high worldwide consumption of beer and the low cost of production, unfiltered beer brewed using our genetically modified <ins class="diffchange diffchange-inline">''</ins>S. cerevisiae<ins class="diffchange diffchange-inline">'' </ins>should provide a cost-effective source of pharmacologically-active resveratrol.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====[[Team:Slovenia | Slovenia]]====</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>====[[Team:Slovenia | Slovenia]]====</div></td></tr>
</table>Msicchio