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Making Frozen Glycerol Cell Stocks From Overnight Cultures
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* Take 2mls of ON culture and pipette into a sterilised cryo tube. | * Take 2mls of ON culture and pipette into a sterilised cryo tube. | ||
* Add 0.5ml of autoclaved 50% glycerol solution | * Add 0.5ml of autoclaved 50% glycerol solution | ||
* Vortex briefly to mix | * Vortex briefly to mix | ||
* Store at -80˚C | * Store at -80˚C | ||
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Latest revision as of 16:06, 28 October 2008
Newcastle University
GOLD MEDAL WINNER 2008
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Home >> Wet Lab >> Protocols >> Making Frozen Glycerol Cell Stocks From Overnight Cultures
- Take 2mls of ON culture and pipette into a sterilised cryo tube.
- Add 0.5ml of autoclaved 50% glycerol solution
- Vortex briefly to mix
- Store at -80˚C
