Making Frozen Glycerol Cell Stocks From Overnight Cultures

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Home >> Wet Lab >> Protocols >> Making Frozen Glycerol Cell Stocks From Overnight Cultures

  • Take 2mls of ON culture and pipette into a sterilised cryo tube.
  • Add 0.5ml of autoclaved 50% glycerol solution
  • Vortex briefly to mix
  • Store at -80˚C
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