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Making Frozen Glycerol Cell Stocks From Overnight Cultures
From 2008.igem.org
Newcastle University
GOLD MEDAL WINNER 2008
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Home >> Wet Lab >> Protocols >> Making Frozen Glycerol Cell Stocks From Overnight Cultures
- Take 2mls of ON culture and pipette into a sterilised cryo tube.
- Add 0.5ml of autoclaved 50% glycerol solution
- Vortex briefly to mix
- Store at -80˚C
