Making Overnight Cultures from Frozen Glycerol Cell Stocks
From 2008.igem.org
(Difference between revisions)
Riachalder (Talk | contribs) |
|||
Line 1: | Line 1: | ||
+ | {{:Team:Newcastle University/Header}} | ||
+ | {{:Team:Newcastle University/Template:UnderTheProtocol|page-title=[[Making Overnight Cultures from Frozen Glycerol Cell Stocks]]}} | ||
+ | |||
[[Image:DSC01644.jpg|thumb|right|300px|Preparing overnight cultures]] | [[Image:DSC01644.jpg|thumb|right|300px|Preparing overnight cultures]] | ||
* Pippette 10mL LB into a red-capped culture tube or conical shake flask. | * Pippette 10mL LB into a red-capped culture tube or conical shake flask. | ||
* Using an autoclaved toothpick, take a scraping of the frozen glycerol stock and drop it into the tube/flask. (Alternatively a pipette tip can be used and discarded after transferring the cells to the LB.) | * Using an autoclaved toothpick, take a scraping of the frozen glycerol stock and drop it into the tube/flask. (Alternatively a pipette tip can be used and discarded after transferring the cells to the LB.) | ||
* Incubate at 37°C overnight. | * Incubate at 37°C overnight. | ||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- | |||
- |
Latest revision as of 16:07, 28 October 2008
Newcastle University
GOLD MEDAL WINNER 2008
Home | Team | Original Aims | Software | Modelling | Proof of Concept Brick | Wet Lab | Conclusions |
---|
Home >> Wet Lab >> Protocols >> Making Overnight Cultures from Frozen Glycerol Cell Stocks
- Pippette 10mL LB into a red-capped culture tube or conical shake flask.
- Using an autoclaved toothpick, take a scraping of the frozen glycerol stock and drop it into the tube/flask. (Alternatively a pipette tip can be used and discarded after transferring the cells to the LB.)
- Incubate at 37°C overnight.