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Making Overnight Cultures from Frozen Glycerol Cell Stocks
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* Using an autoclaved toothpick, take a scraping of the frozen glycerol stock and drop it into the tube/flask. (Alternatively a pipette tip can be used and discarded after transferring the cells to the LB.) | * Using an autoclaved toothpick, take a scraping of the frozen glycerol stock and drop it into the tube/flask. (Alternatively a pipette tip can be used and discarded after transferring the cells to the LB.) | ||
* Incubate at 37°C overnight. | * Incubate at 37°C overnight. | ||
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Revision as of 10:52, 18 September 2008
- Pippette 10mL LB into a red-capped culture tube or conical shake flask.
- Using an autoclaved toothpick, take a scraping of the frozen glycerol stock and drop it into the tube/flask. (Alternatively a pipette tip can be used and discarded after transferring the cells to the LB.)
- Incubate at 37°C overnight.
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