Minnesota/16 July 2008

From 2008.igem.org

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|2. '''Move Dual Promoter Plates from incubator to fridge'''. This will stop over growth and mutations before picking into 2mL cultures is performed later on.
|2. '''Move Dual Promoter Plates from incubator to fridge'''. This will stop over growth and mutations before picking into 2mL cultures is performed later on.
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|3. '''Transform paper DNA from iGEM notebook:''' (1) RFP, (2) TetR promoter, (3) Terminator, (4) MCherry. Follow iGEM handout for details on paper transformations into DH5alpha competent cells.  
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|3. '''Transform paper DNA from iGEM notebook:''' (1) RFP, (2) TetR promoter, (3) Terminator, (4) MCherry. Follow iGEM handout for details on paper transformations into TOP10 competent cells.  
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|[[Image:TransformationTetR.jpg|thumb|left|600px|Example of Transformation using TetR Promoter Gene]]
|[[Image:TransformationTetR.jpg|thumb|left|600px|Example of Transformation using TetR Promoter Gene]]
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|4. '''Sequencing Results:''' Sequencing results returned poor results. Redo/rework on sequences.
|4. '''Sequencing Results:''' Sequencing results returned poor results. Redo/rework on sequences.

Latest revision as of 20:34, 5 August 2008

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1. Plasmid Prep 2mL Ligated Product cultures from 07-14-2008. Follow the QIA prep and purify steps as seen in on 07-14-2008.
2. Move Dual Promoter Plates from incubator to fridge. This will stop over growth and mutations before picking into 2mL cultures is performed later on.
3. Transform paper DNA from iGEM notebook: (1) RFP, (2) TetR promoter, (3) Terminator, (4) MCherry. Follow iGEM handout for details on paper transformations into TOP10 competent cells.
Example of Transformation using TetR Promoter Gene
4. Sequencing Results: Sequencing results returned poor results. Redo/rework on sequences.