Newcastle University Wetlab/10 September 2008
From 2008.igem.org
Newcastle University
GOLD MEDAL WINNER 2008
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Wet lab work was carried out from 4 August to 19 September, Mondays to Fridays. Please click on a day to see the lab notebook.
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Wednesday 10th September
- Overnight ligation mixtures transformed into competent DH5α E. coli cells (see protocol).
- 12.5μl pGFPrrnB-BBa_746107 + 100μl DH5α cells - 12.5μl pGFPrrnB + 100μl DH5α cells (positive control) - 100μl DH5α cells (negative control)
- All transformants were then plated onto spectinomycin agar in both 25μl and 250μl volumes and incubated overnight at 37˚C.