Newcastle University Wetlab/12 August 2008

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(New page: {{:Team:Newcastle_University/WetLabCalendarHeader}} ==Tuesday 12th August== * pGFPrrnB and pJWV021 were restricted using 10μl plasmid and 0.5μl each enzyme in 50μl total reaction ...)
 
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==Tuesday 12th August==
==Tuesday 12th August==
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* pGFPrrnB and pJWV021 were [[restricted]] using 10μl plasmid and 0.5μl each enzyme in 50μl total reaction volume:
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* pGFPrrnB and pJWV021 were [[Restricting Plasmids (Double Restriction)|restricted]] using 10μl plasmid and 0.5μl each enzyme in 50μl total reaction volume:
- pGFPrrnB restricted with EcoRI + NheI
- pGFPrrnB restricted with EcoRI + NheI
- pJWV021 restricted with NheI then BglII in a 2 step reaction (the second step used 48μl purified plasmid in 100μl total reaction volume).
- pJWV021 restricted with NheI then BglII in a 2 step reaction (the second step used 48μl purified plasmid in 100μl total reaction volume).
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Running the samples on a [[gel]] showed only partial restriction had occurred and that there was very little pJWV021.
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Running the samples on a [[Agarose Gel Electrophoresis|gel]] showed only partial restriction had occurred and that there was very little pJWV021.

Latest revision as of 16:28, 28 October 2008

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Wet lab work was carried out from 4 August to 19 September, Mondays to Fridays. Please click on a day to see the lab notebook.

August
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September
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Tuesday 12th August

  • pGFPrrnB and pJWV021 were restricted using 10μl plasmid and 0.5μl each enzyme in 50μl total reaction volume:

- pGFPrrnB restricted with EcoRI + NheI

- pJWV021 restricted with NheI then BglII in a 2 step reaction (the second step used 48μl purified plasmid in 100μl total reaction volume).

Running the samples on a gel showed only partial restriction had occurred and that there was very little pJWV021.