Newcastle University Wetlab/14 August 2008

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(New page: {{:Team:Newcastle_University/WetLabCalendarHeader}} ==Thursday 14th August== * BBa_I746101 and BBa_746107 were isolated and run on a gel together with the 4 restrictions performe...)
 
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==Thursday 14th August==
==Thursday 14th August==
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* BBa_I746101 and BBa_746107 were [[isolated]] and run on a [[gel]] together with the 4 restrictions performed 13.08.08.
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* BBa_I746101 and BBa_746107 were [[Isolating Plasmid from Cells (Miniprep)|isolated]] and run on a [[Agarose Gel Electrophoresis|gel]] together with the 4 restrictions performed 13.08.08.
'''Lane 1:''' 1kb ladder
'''Lane 1:''' 1kb ladder

Latest revision as of 16:27, 28 October 2008

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Newcastle University

GOLD MEDAL WINNER 2008

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Home >> Wet Lab >> Newcastle Wet Lab Journal

Wet lab work was carried out from 4 August to 19 September, Mondays to Fridays. Please click on a day to see the lab notebook.

August
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September
MTWTFSS
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Thursday 14th August

  • BBa_I746101 and BBa_746107 were isolated and run on a gel together with the 4 restrictions performed 13.08.08.

Lane 1: 1kb ladder

Lane 2: empty

Lane 3: pGFPrrnB restricted with EcoRI + NheI

Lane 4: pJWV021 restricted with NheI + BglII

Lane 5: pUC57-ncl08 restricted with EcoRI + NheI

Lane 6: pUC57-ncl08 restricted with NheI + BglII

Lane 7: BBa_746101

Lane 8: BBa_746107

The gel showed that there was very little pGFPrrnB (restricted with EcoRI + NheI) and no pJWV021. It was concluded that the 2 step restriction and purification method reduced the plasmid yield significantly. As it was later discovered that this method was unnecessary (as NheI and BglII are compatible using buffer M), it was decided to perform the restriction as a single step in future.

The gel also showed successful restrictions of the pUC57-ncl08 plasmid and lots of the two BioBrick samples.