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Newcastle University Wetlab/15 August 2008
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(New page: {{:Team:Newcastle_University/WetLabCalendarHeader}} ==Friday 15th August== pGFPrrnB, pJWV021 and BBa_I746107 were restricted using 20μl plasmid and 1μl each enzyme in 40μl total r...) |
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==Friday 15th August== | ==Friday 15th August== | ||
| - | pGFPrrnB, pJWV021 and BBa_I746107 were [[restricted]] using 20μl plasmid and 1μl each enzyme in 40μl total reaction volume: | + | pGFPrrnB, pJWV021 and BBa_I746107 were [[Restricting Plasmids (Double Restriction)|restricted]] using 20μl plasmid and 1μl each enzyme in 40μl total reaction volume: |
- pGFPrrnB restricted with EcoRI + NheI | - pGFPrrnB restricted with EcoRI + NheI | ||
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- BBa_I746107 restricted with EcoRI + SpeI | - BBa_I746107 restricted with EcoRI + SpeI | ||
| - | These were incubated at 37˚C for 1 hour and run on a [[gel]]. The gel showed that pGFPrrnB and pJWV021 had restricted well with EcoRI + NheI and Nhe +BglII respectively, but pGFPrrnB and BBa_I746107 restricted with EcoRI + SpeI had only partially restricted. | + | These were incubated at 37˚C for 1 hour and run on a [[Agarose Gel Electrophoresis|gel]]. The gel showed that pGFPrrnB and pJWV021 had restricted well with EcoRI + NheI and Nhe +BglII respectively, but pGFPrrnB and BBa_I746107 restricted with EcoRI + SpeI had only partially restricted. |
Latest revision as of 16:26, 28 October 2008
Newcastle University
GOLD MEDAL WINNER 2008
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Wet lab work was carried out from 4 August to 19 September, Mondays to Fridays. Please click on a day to see the lab notebook.
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Friday 15th August
pGFPrrnB, pJWV021 and BBa_I746107 were restricted using 20μl plasmid and 1μl each enzyme in 40μl total reaction volume: - pGFPrrnB restricted with EcoRI + NheI
- pJWV021 restricted with NheI + BglII
- pGFPrrnB restricted with EcoRI + SpeI
- BBa_I746107 restricted with EcoRI + SpeI
These were incubated at 37˚C for 1 hour and run on a gel. The gel showed that pGFPrrnB and pJWV021 had restricted well with EcoRI + NheI and Nhe +BglII respectively, but pGFPrrnB and BBa_I746107 restricted with EcoRI + SpeI had only partially restricted.
