Newcastle University Wetlab/15 August 2008
From 2008.igem.org
Newcastle University
GOLD MEDAL WINNER 2008
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Wet lab work was carried out from 4 August to 19 September, Mondays to Fridays. Please click on a day to see the lab notebook.
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Friday 15th August
pGFPrrnB, pJWV021 and BBa_I746107 were restricted using 20μl plasmid and 1μl each enzyme in 40μl total reaction volume: - pGFPrrnB restricted with EcoRI + NheI
- pJWV021 restricted with NheI + BglII
- pGFPrrnB restricted with EcoRI + SpeI
- BBa_I746107 restricted with EcoRI + SpeI
These were incubated at 37˚C for 1 hour and run on a gel. The gel showed that pGFPrrnB and pJWV021 had restricted well with EcoRI + NheI and Nhe +BglII respectively, but pGFPrrnB and BBa_I746107 restricted with EcoRI + SpeI had only partially restricted.