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Newcastle University Wetlab/22 August 2008
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(New page: {{:Team:Newcastle_University/WetLabCalendarHeader}} ==Friday 22nd August== * Stock pGFPrrnB from -80˚C was plated onto ampicillin, spectinomycin and normal agar (control) and incuba...) |
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| - | * Stock pGFPrrnB from -80˚C was plated onto ampicillin, spectinomycin and normal [[agar]] (control) and incubated overnight at 37˚C. | + | * Stock pGFPrrnB from -80˚C was plated onto ampicillin, spectinomycin and normal [[Making Agar Plates|agar]] (control) and incubated overnight at 37˚C. |
| - | * 2μl pUC57-ncl08 and pJWV021 were run on [[gel]] to check quantity. This showed large quantities of both plasmids and therefore pUC57-ncl08 was [[restricted]] with EcoRI + NHeI and NHeI + BglII. Controls (buffer A and buffer M without enzyme) were used for both restrictions. | + | * 2μl pUC57-ncl08 and pJWV021 were run on [[Agarose Gel Electrophoresis|gel]] to check quantity. This showed large quantities of both plasmids and therefore pUC57-ncl08 was [[Restricting Plasmids (Double Restriction)|restricted]] with EcoRI + NHeI and NHeI + BglII. Controls (buffer A and buffer M without enzyme) were used for both restrictions. |
Latest revision as of 16:24, 28 October 2008
Newcastle University
GOLD MEDAL WINNER 2008
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Home >> Wet Lab >> Newcastle Wet Lab Journal
Wet lab work was carried out from 4 August to 19 September, Mondays to Fridays. Please click on a day to see the lab notebook.
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Friday 22nd August
- Stock pGFPrrnB from -80˚C was plated onto ampicillin, spectinomycin and normal agar (control) and incubated overnight at 37˚C.
- 2μl pUC57-ncl08 and pJWV021 were run on gel to check quantity. This showed large quantities of both plasmids and therefore pUC57-ncl08 was restricted with EcoRI + NHeI and NHeI + BglII. Controls (buffer A and buffer M without enzyme) were used for both restrictions.
