http://2008.igem.org/wiki/index.php?title=Newcastle_University_Wetlab/26_August_2008&feed=atom&action=historyNewcastle University Wetlab/26 August 2008 - Revision history2024-03-29T11:47:18ZRevision history for this page on the wikiMediaWiki 1.16.5http://2008.igem.org/wiki/index.php?title=Newcastle_University_Wetlab/26_August_2008&diff=47248&oldid=prevMtaschuk at 18:34, 21 September 20082008-09-21T18:34:19Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Lane 7:''' pure pJWV021</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>'''Lane 7:''' pure pJWV021</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[[Image:DSC01658f.jpg<del class="diffchange diffchange-inline">|thumb|left</del>|200px|pGFP-rrnB colonies on +Spec agar plate]]</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[[Image:DSC01658f.jpg|200px|pGFP-rrnB colonies on +Spec agar plate]]</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Results from overnight (ON) agar cultures:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Results from overnight (ON) agar cultures:</div></td></tr>
</table>Mtaschukhttp://2008.igem.org/wiki/index.php?title=Newcastle_University_Wetlab/26_August_2008&diff=47247&oldid=prevMtaschuk: New page: {{:Team:Newcastle_University/WetLabCalendarHeader}} ==Tuesday 26th August== * Due to unforeseen circumstances, restriction mixtures were put in the fridge on Friday 22.08.08. Therefore t...2008-09-21T18:33:17Z<p>New page: {{:Team:Newcastle_University/WetLabCalendarHeader}} ==Tuesday 26th August== * Due to unforeseen circumstances, restriction mixtures were put in the fridge on Friday 22.08.08. Therefore t...</p>
<p><b>New page</b></p><div>{{:Team:Newcastle_University/WetLabCalendarHeader}}<br />
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==Tuesday 26th August==<br />
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* Due to unforeseen circumstances, restriction mixtures were put in the fridge on Friday 22.08.08. Therefore the restriction was allowed to run for a further 1.5 hours before running on a gel.<br />
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[[Image:Jelly 26-08-081.jpg|thumb|right|300px|Gel showing our pUC57-ncl08 restrictions plus controls]]<br />
'''Lane 1:''' 1kb ladder<br />
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'''Lane 2:''' empty<br />
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'''Lane 3:''' pUC57-ncl08 restricted with EcoRI + NHeI<br />
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'''Lane 4:''' pUC57-ncl08 restricted with NHeI and BglII<br />
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'''Lane 5:''' pUC57-ncl08 buffer A control<br />
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'''Lane 6:''' pUC57-ncl08 buffer M control<br />
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'''Lane 7:''' pure pJWV021<br />
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[[Image:DSC01658f.jpg|thumb|left|200px|pGFP-rrnB colonies on +Spec agar plate]]<br />
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Results from overnight (ON) agar cultures:<br />
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- pGFPrrnB (amp) - no colonies<br />
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- pGFPrrnB (spec) - colonies present<br />
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- pGFPrrnB (no antibiotic) - colonies present<br />
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* ON cultures were made:<br />
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- pGFP-rrnB grown on ampicillin<br />
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- pJWV021 grown on ampicillin<br />
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- pUC57-ncl08 grown on ampicillin</div>Mtaschuk