Newcastle University Wetlab/29 August 2008

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Wet lab work was carried out from 4 August to 19 September, Mondays to Fridays. Please click on a day to see the lab notebook.

August
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September
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Friday 29th August

  • Today we restricted our pUC57-ncl08 plasmid. In one reaction we used EcoR1 and Nhe1, and in other we used BglII and Nhe1. We also carried out a restriction with just BglII to check for the correct sized linear DNA. In addition we ran unrestricted plasmid on the gel.
  Expected band sizes:
              * For digestion with BglII only: ~ 4.9kb
              * For digestion with Nhe1 and EcoR1: ~ 2.2kb and 2.7kb
              * For digestion with Nhe1 and BglII: ~ 2.2kb and 2.7kb

Gel showing successful digestions of pUC57-ncl08

  • As you can see, the restrictions appear successful.