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| - | {{Paris/Calendar}}
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| - | [[Paris/July 12|< Yesterday]] - [[Paris/July 14|Tomorrow >]]
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| - | == Preparation of Competent Cells ==
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| - | * Dilution 1/200e (1mL of culture on 200mL of LB)
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| - | * Culture at 37°C until DO=0,57 (around 3h)
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| - | * Sharing into 4 Falcon tubes of 50mL and cooling at 4°C during 40' in ice
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| - | * Spin during 8', 2000rpm, 4°C
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| - | * Resuspension of the cell's cap in 20mL of CaCl2 (0,1M) cooled at 4°C
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Latest revision as of 08:43, 16 July 2008
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