From 2008.igem.org
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| - | <div style="display: inline; border: thin solid black; background: yellow">[[Paris/July 12|Yesterday]]</div>
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| - | <div style="display: inline; border: thin solid black; background: yellow">[[Paris/July 14|Tomorrow >]]</div>
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| - | {{Paris/Calendar_Inline}}
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| - | [[Preparation of Competent Cells]]
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| - | * Dilution 1/200e (1mL of the O/N culture on 200mL of LB)
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| - | * Culture at 37°C until DO=0,57 (around 3h)
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| - | * Sharing into 4 Falcon tubes of 50mL and cooling at 4°C during 40' in ice
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| - | * Spin during 8', 2000rpm, 4°C
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| - | * Resuspension of the cell's pellet in 20mL of cold CaCl2 (0,1M) during 30' in ice
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| - | * Spin during 8', 2000rpm, 4°C
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| - | * Resuspension of the cell's pellet in 1mL of cold CaCl2 (0,1M)
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| - | * 1h of delay unexpected (problem with DNA lyophilized)
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| - | * Transformation of the Biobricks with the "classical" protocol (--> heat shock at 42°C durin 40")
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| - | * Incubation O/N at 37°C of the transformed bacteria
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| - | [[Remark :]]
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| - | * IGEM 2008 Biobricks : use of the maximum recoverable volume minipreps
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| - | * IGEM 2007 Biobricks : use of 3µl of minipreps
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Latest revision as of 08:43, 16 July 2008
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