From 2008.igem.org
(Difference between revisions)
|
|
| (16 intermediate revisions not shown) |
| Line 1: |
Line 1: |
| - | <center><div style="display: inline; border: thin solid black; background: yellow; color: red;">[[Paris/July 12| < Yesterday]]</div> - <div style="display: inline; border: thin solid black; background: yellow; color: red;">[[Paris/July 14|Tomorrow > ]]</div></center>
| + | delete |
| - | {{Paris/Calendar_Inline}}
| + | |
| - | | + | |
| - | | + | |
| - | [[Preparation of Competent Cells]]
| + | |
| - | | + | |
| - | * Dilution 1/200e (1mL of the O/N culture on 200mL of LB)
| + | |
| - | * Culture at 37°C until DO=0,57 (around 3h)
| + | |
| - | * Sharing into 4 Falcon tubes of 50mL and cooling at 4°C during 40' in ice
| + | |
| - | * Spin during 8', 2000rpm, 4°C
| + | |
| - | * Resuspension of the cell's pellet in 20mL of cold CaCl2 (0,1M) during 30' in ice
| + | |
| - | * Spin during 8', 2000rpm, 4°C
| + | |
| - | * Resuspension of the cell's pellet in 1mL of cold CaCl2 (0,1M)
| + | |
| - | * 1h of delay unexpected (problem with DNA lyophilized)
| + | |
| - | * Transformation of the Biobricks with the "classical" protocol (--> heat shock at 42°C durin 40")
| + | |
| - | * Incubation O/N at 37°C of the transformed bacteria
| + | |
| - | | + | |
| - | | + | |
| - | [[Remark :]]
| + | |
| - | | + | |
| - | * IGEM 2008 Biobricks : use of the maximum recoverable volume minipreps
| + | |
| - | * IGEM 2007 Biobricks : use of 3µl of minipreps
| + | |
| - | | + | |
| - | | + | |
| - | | + | |
| - | | + | |
| - | {| border="1"
| + | |
| - | |align="center"|'''details'''
| + | |
| - | |align="center"|''' '''
| + | |
| - | |align="center"|'''resitance'''
| + | |
| - | |align="center"|'''Plate 2007'''
| + | |
| - | |-
| + | |
| - | |2E
| + | |
| - | |SP 1000
| + | |
| - | |B0034 (A)
| + | |
| - | |3O P1
| + | |
| - | |-
| + | |
| - | |10F
| + | |
| - | |SP 1001
| + | |
| - | |R0079 (A)
| + | |
| - | |19N P1
| + | |
| - | |-
| + | |
| - | |4F
| + | |
| - | |SP 1013
| + | |
| - | |C0079(K)
| + | |
| - | |7K P2
| + | |
| - | |-
| + | |
| - | |6H
| + | |
| - | |SP 1001
| + | |
| - | |C0179 (A)
| + | |
| - | |6H P1
| + | |
| - | |-
| + | |
| - | |6D
| + | |
| - | |SP 1007
| + | |
| - | |S03879 (A)
| + | |
| - | | /
| + | |
| - | |-
| + | |
| - | |6B
| + | |
| - | |SP 1001
| + | |
| - | |S03154 (A)
| + | |
| - | |24K P1
| + | |
| - | |-
| + | |
| - | |10F
| + | |
| - | |SP 1002
| + | |
| - | |J23101 (A)
| + | |
| - | |21G P3
| + | |
| - | |-
| + | |
| - | |11F
| + | |
| - | |SP 1002
| + | |
| - | |J23109 (A)
| + | |
| - | |23G P3
| + | |
| - | |-
| + | |
| - | |4C
| + | |
| - | |SP 1000
| + | |
| - | |R0040 (A)
| + | |
| - | |7O P1
| + | |
| - | |-
| + | |
| - | |2G
| + | |
| - | |SP 1000
| + | |
| - | |C0040 (A)
| + | |
| - | |5C P1
| + | |
| - | |-
| + | |
| - | |1C
| + | |
| - | |SP 1008
| + | |
| - | |PSB1A3 (A)
| + | |
| - | |23E P1
| + | |
| - | |-
| + | |
| - | |7G
| + | |
| - | |SP 1013
| + | |
| - | |E1010 (K)
| + | |
| - | |15M P2
| + | |
| - | |-
| + | |
| - | |7E
| + | |
| - | |SP 1001
| + | |
| - | |E0040 (A)
| + | |
| - | |5H P1
| + | |
| - | |-
| + | |
| - | |2F
| + | |
| - | |SP 1016
| + | |
| - | |E0030 (K)
| + | |
| - | |7G P1
| + | |
| - | |}
| + | |
Latest revision as of 08:43, 16 July 2008
delete
"
