Paris/July 13

From 2008.igem.org

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<center><div style="display: inline; border: thin solid black; background: yellow; color: red;">[[Paris/July 12|&nbsp;< Yesterday]]</div> - <div style="display: inline; border: thin solid black; background: yellow; color: red;">[[Paris/July 14|Tomorrow >&nbsp;]]</div></center>
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{{Paris/Calendar_Inline}}
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[[Preparation of Competent Cells]]
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* Dilution 1/200e (1mL of the O/N culture on 200mL of LB)
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* Culture at 37°C until DO=0,57 (around 3h)
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* Sharing into 4 Falcon tubes of 50mL and cooling at 4°C during 40' in ice
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* Spin during 8', 2000rpm, 4°C
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* Resuspension of the cell's pellet in 20mL of cold CaCl2 (0,1M) during 30' in ice
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* Spin during 8', 2000rpm, 4°C
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* Resuspension of the cell's pellet in 1mL of cold CaCl2 (0,1M)
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* 1h of delay unexpected (problem with DNA lyophilized)
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* Transformation of the Biobricks with the "classical" protocol (--> heat shock at 42°C durin 40")
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* Incubation O/N at 37°C of the transformed bacteria
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[[Remark :]]
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* Biobricks 2008 : use of the maximum recoverable volume minipreps
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* Biobricks 2007 : use of 3µl of minipreps
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{| border="1"
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|colspan="2" |'''Biobricks 2007'''
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|align="center"|Strain
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|align="center"|Transformation
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|-
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|MP1 (= J23100)
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|ok
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|-
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|MP2 (= J23107)
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|ok
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|-
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|MP3 (= B0015)
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|ok
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|-
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|MP5 (= JB0030)
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|ok
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|-
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|MP6 (= E0422)
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|ok
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|}
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{| border="1"
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|colspan="4" |'''Biobricks 2008'''
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|align="center"|details
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|align="center"|Strain
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|align="center"|Antibiotics
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|align="center"|Plate 2007
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|-
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|2E_SP 1000
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|B0034
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|A
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|3O P1
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|-
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|10F_SP 1001
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|R0079
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|A
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|19N P1
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|-
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|4F_SP 1013
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|C0079
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|K
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|7K P2
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|-
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|6H_SP 1001
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|C0179
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|A
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|6H P1
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|-
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|6D_SP 1007
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|S03879
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|A
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| /
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|-
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|6B_SP 1001
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|S03154
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|A
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|24K P1
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|-
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|10F_SP 1002
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|J23101
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|A
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|21G P3
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|-
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|11F_SP 1002
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|J23109
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|A
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|23G P3
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|-
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|4C_SP 1000
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|R0040
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|A
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|7O P1
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|-
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|2G_SP 1000
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|C0040
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|A
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|5C P1
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|-
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|1C_SP 1008
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|PSB1A3
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|A
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|23E P1
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|-
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|7G_SP 1013
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|E1010
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|K
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|15M P2
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|-
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|7E_SP 1001
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|E0040
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|A
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|5H P1
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|-
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|2F_SP 1016
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|E0030
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|K
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|7G P1
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|}
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Latest revision as of 08:43, 16 July 2008

delete

Retrieved from "http://2008.igem.org/Paris/July_13"