Paris/July 13

From 2008.igem.org

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Latest revision as of 08:43, 16 July 2008

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-<center><div style="display: inline; border: thin solid black; background: yellow; color: red;">[[Paris/July 12|&nbsp;< Yesterday]]</div> - <div style="display: inline; border: thin solid black; background: yellow; color: red;">[[Paris/July 14|Tomorrow >&nbsp;]]</div></center>
+delete
-{{Paris/Calendar_Inline}}
-[[Preparation of Competent Cells]]
-* Dilution 1/200e (1mL of the O/N culture on 200mL of LB)
-* Culture at 37°C until DO=0,57 (around 3h)
-* Sharing into 4 Falcon tubes of 50mL and cooling at 4°C during 40' in ice
-* Spin during 8', 2000rpm, 4°C
-* Resuspension of the cell's pellet in 20mL of cold CaCl2 (0,1M) during 30' in ice
-* Spin during 8', 2000rpm, 4°C
-* Resuspension of the cell's pellet in 1mL of cold CaCl2 (0,1M)
-* 1h of delay unexpected (problem with DNA lyophilized)
-* Transformation of the Biobricks with the "classical" protocol (--> heat shock at 42°C during 40")
-* Incubation O/N at 37°C of the transformed bacteria
-[[Remark :]]
-* Biobricks 2008 : use of the maximum recoverable volume of the minipreps
-* Biobricks 2007 : use of 3µl of the minipreps
-[[Liste of the transformation:]]
-'''Biobricks 2007'''
-{| border="1"
-|align="center"|Strain
-|align="center"|Transformation
-|-
-|MP1 (= J23100)
-|align="center"|ok
-|-
-|MP2 (= J23107)
-|align="center"|ok
-|-
-|MP3 (= B0015)
-|align="center"|ok
-|-
-|MP5 (= JB0030)
-|align="center"|ok
-|-
-|MP6 (= E0422)
-|align="center"|ok
-|}
-'''Biobricks 2008'''
-{| border="1"
-|align="center"|details
-|align="center"|Strain
-|align="center"|Antibio
-|align="center"|Plate 2007
-|-
-|align="center"|2E_SP 1000
-|align="center"|B0034
-|align="center"|A
-|align="center"|3O P1
-|-
-|align="center"|10F_SP 1001
-|align="center"|R0079
-|align="center"|A
-|align="center"|19N P1
-|-
-|align="center"|4F_SP 1013
-|align="center"|C0079
-|align="center"|K
-|align="center"|7K P2
-|-
-|align="center"|6H_SP 1001
-|align="center"|C0179
-|align="center"|A
-|align="center"|6H P1
-|-
-|align="center"|6D_SP 1007
-|align="center"|S03879
-|align="center"|A
-|align="center"| /
-|-
-|align="center"|6B_SP 1001
-|align="center"|S03154
-|align="center"|A
-|align="center"|24K P1
-|-
-|align="center"|10F_SP 1002
-|align="center"|J23101
-|align="center"|A
-|align="center"|21G P3
-|-
-|align="center"|11F_SP 1002
-|align="center"|J23109
-|align="center"|A
-|align="center"|23G P3
-|-
-|align="center"|4C_SP 1000
-|align="center"|R0040
-|align="center"|A
-|align="center"|7O P1
-|-
-|align="center"|2G_SP 1000
-|align="center"|C0040
-|align="center"|A
-|align="center"|5C P1
-|-
-|align="center"|1C_SP 1008
-|align="center"|PSB1A3
-|align="center"|A
-|align="center"|23E P1
-|-
-|align="center"|7G_SP 1013
-|align="center"|E1010
-|align="center"|K
-|align="center"|15M P2
-|-
-|align="center"|7E_SP 1001
-|align="center"|E0040
-|align="center"|A
-|align="center"|5H P1
-|-
-|align="center"|2F_SP 1016
-|align="center"|E0030
-|align="center"|K
-|align="center"|7G P1
-|}