|
|
(7 intermediate revisions not shown) |
Line 1: |
Line 1: |
- | <center><div style="display: inline; border: thin solid black; background: yellow; color: red;">[[Paris/July 12| < Yesterday]]</div> - <div style="display: inline; border: thin solid black; background: yellow; color: red;">[[Paris/July 14|Tomorrow > ]]</div></center>
| + | delete |
- | {{Paris/Calendar_Inline}}
| + | |
- | | + | |
- | | + | |
- | [[Preparation of Competent Cells]]
| + | |
- | | + | |
- | * Dilution 1/200e (1mL of the O/N culture on 200mL of LB)
| + | |
- | * Culture at 37°C until DO=0,57 (around 3h)
| + | |
- | * Sharing into 4 Falcon tubes of 50mL and cooling at 4°C during 40' in ice
| + | |
- | * Spin during 8', 2000rpm, 4°C
| + | |
- | * Resuspension of the cell's pellet in 20mL of cold CaCl2 (0,1M) during 30' in ice
| + | |
- | * Spin during 8', 2000rpm, 4°C
| + | |
- | * Resuspension of the cell's pellet in 1mL of cold CaCl2 (0,1M)
| + | |
- | * 1h of delay unexpected (problem with DNA lyophilized)
| + | |
- | * Transformation of the Biobricks with the "classical" protocol (--> heat shock at 42°C during 40")
| + | |
- | * Incubation O/N at 37°C of the transformed bacteria
| + | |
- | | + | |
- | | + | |
- | [[Remark :]]
| + | |
- | | + | |
- | * Biobricks 2007 : use of 3µl of the minipreps
| + | |
- | * Biobricks 2008 : use of the maximum recoverable volume of the minipreps
| + | |
- | | + | |
- | | + | |
- | | + | |
- | [[Liste of the transformation:]]
| + | |
- | | + | |
- | *'''Biobricks 2007'''
| + | |
- | {| border="1"
| + | |
- | |align="center"|Strain
| + | |
- | |align="center"|Transformation
| + | |
- | |-
| + | |
- | |MP1 (= J23100)
| + | |
- | |align="center"|ok
| + | |
- | |-
| + | |
- | |MP2 (= J23107)
| + | |
- | |align="center"|ok
| + | |
- | |-
| + | |
- | |MP3 (= B0015)
| + | |
- | |align="center"|ok
| + | |
- | |-
| + | |
- | |MP5 (= JB0030)
| + | |
- | |align="center"|ok
| + | |
- | |-
| + | |
- | |MP6 (= E0422)
| + | |
- | |align="center"|ok
| + | |
- | |}
| + | |
- | | + | |
- | *'''Biobricks 2008'''
| + | |
- | {| border="1"
| + | |
- | |align="center"|details
| + | |
- | |align="center"|Strain
| + | |
- | |align="center"|Antibio
| + | |
- | |align="center"|Plate 2007
| + | |
- | |-
| + | |
- | |align="center"|2E_SP 1000
| + | |
- | |align="center"|B0034
| + | |
- | |align="center"|A
| + | |
- | |align="center"|3O P1
| + | |
- | |-
| + | |
- | |align="center"|10F_SP 1001
| + | |
- | |align="center"|R0079
| + | |
- | |align="center"|A
| + | |
- | |align="center"|19N P1
| + | |
- | |-
| + | |
- | |align="center"|4F_SP 1013
| + | |
- | |align="center"|C0079
| + | |
- | |align="center"|K
| + | |
- | |align="center"|7K P2
| + | |
- | |-
| + | |
- | |align="center"|6H_SP 1001
| + | |
- | |align="center"|C0179
| + | |
- | |align="center"|A
| + | |
- | |align="center"|6H P1
| + | |
- | |-
| + | |
- | |align="center"|6D_SP 1007
| + | |
- | |align="center"|S03879
| + | |
- | |align="center"|A
| + | |
- | |align="center"| /
| + | |
- | |-
| + | |
- | |align="center"|6B_SP 1001
| + | |
- | |align="center"|S03154
| + | |
- | |align="center"|A
| + | |
- | |align="center"|24K P1
| + | |
- | |-
| + | |
- | |align="center"|10F_SP 1002
| + | |
- | |align="center"|J23101
| + | |
- | |align="center"|A
| + | |
- | |align="center"|21G P3
| + | |
- | |-
| + | |
- | |align="center"|11F_SP 1002
| + | |
- | |align="center"|J23109
| + | |
- | |align="center"|A
| + | |
- | |align="center"|23G P3
| + | |
- | |-
| + | |
- | |align="center"|4C_SP 1000
| + | |
- | |align="center"|R0040
| + | |
- | |align="center"|A
| + | |
- | |align="center"|7O P1
| + | |
- | |-
| + | |
- | |align="center"|2G_SP 1000
| + | |
- | |align="center"|C0040
| + | |
- | |align="center"|A
| + | |
- | |align="center"|5C P1
| + | |
- | |-
| + | |
- | |align="center"|1C_SP 1008
| + | |
- | |align="center"|PSB1A3
| + | |
- | |align="center"|A
| + | |
- | |align="center"|23E P1
| + | |
- | |-
| + | |
- | |align="center"|7G_SP 1013
| + | |
- | |align="center"|E1010
| + | |
- | |align="center"|K
| + | |
- | |align="center"|15M P2
| + | |
- | |-
| + | |
- | |align="center"|7E_SP 1001
| + | |
- | |align="center"|E0040
| + | |
- | |align="center"|A
| + | |
- | |align="center"|5H P1
| + | |
- | |-
| + | |
- | |align="center"|2F_SP 1016
| + | |
- | |align="center"|E0030
| + | |
- | |align="center"|K
| + | |
- | |align="center"|7G P1
| + | |
- | |}
| + | |