Paris/July 13

From 2008.igem.org

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(New page: yesterday -- tomorrow <br> == Preparation of Competent Cells == * Dilution 1/200e (1mL of culture on 200mL of LB) * Culture at 37°C until DO=0,57 (a...)
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[[Paris/July 12|yesterday]] -- [[Paris/July 14|tomorrow]] <br>
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[[Paris/July 12|yesterday]] -- [[Paris/July 14|tomorrow]]

Revision as of 17:09, 15 July 2008

yesterday -- tomorrow


Preparation of Competent Cells

  • Dilution 1/200e (1mL of culture on 200mL of LB)
  • Culture at 37°C until DO=0,57 (around 3h)
  • Sharing into 4 Falcon tubes of 50mL and cooling at 4°C during 40' in ice
  • Spin during 8', 2000rpm, 4°C
  • Resuspension of the cell's cap in 20mL of CaCl2 (0,1M) cooled at 4°C
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