Paris/July 13

From 2008.igem.org

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Revision as of 17:13, 15 July 2008

[< Yesterday][Tomorrow >}


Preparation of Competent Cells

  • Dilution 1/200e (1mL of culture on 200mL of LB)
  • Culture at 37°C until DO=0,57 (around 3h)
  • Sharing into 4 Falcon tubes of 50mL and cooling at 4°C during 40' in ice
  • Spin during 8', 2000rpm, 4°C
  • Resuspension of the cell's cap in 20mL of CaCl2 (0,1M) cooled at 4°C
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