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Paris/July 13
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Preparation of Competent Cells
- Dilution 1/200e (1mL of culture on 200mL of LB)
- Culture at 37°C until DO=0,57 (around 3h)
- Sharing into 4 Falcon tubes of 50mL and cooling at 4°C during 40' in ice
- Spin during 8', 2000rpm, 4°C
- Resuspension of the cell's cap in 20mL of CaCl2 (0,1M) cooled at 4°C
