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Protocols E.coliTransformation
From 2008.igem.org
Revision as of 17:37, 29 October 2008 by RAUL CUERO (Talk | contribs)
Cocktail:
70 ul d.s.H2O
10 ul Ligation product
100 ul Competent cells
___________________
180 ul Total
Control:
70ul d.s.H2O
10ul ligation product
100ul Competent cells
___________________
180ul Total
Procedure:
Let product sit on ice for 10 minutes
Take out plates with corresponding antibiotics
Label plates
Heat shock in 42*C water bath for 1.5 minutes
Put on ice for 5 minutes
Turn on Bunsen Burner
Pipette all cells and put on plate
Spread cells on plate with streaks for cell/colony dillution
Put in 37*C incubator lid facing down for 8 hours or overnight
